Modification of cellulose thin films with lysine moieties: a promising approach to achieve antifouling performance

Thomas Elschner; Matej Bračič; Tamilselvan Mohan; Rupert Kargl; Karin Stana Kleinschek

doi:10.1007/s10570-017-1538-9

Modification of cellulose thin films with lysine moieties: a promising approach to achieve antifouling performance

Thomas Elschner, Matej Bračič, Tamilselvan Mohan, Rupert Kargl, Karin Stana Kleinschek^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Thin films of trimethylsilyl cellulose are obtained by spin coating and regenerated to cellulose. The surface is activated with N,N′-carbonyldiimidazole and aminolysis with lysine is carried out in aqueous solution to yield a cellulose lysine carbamate film. The surface is analytically characterized by ATR-IR spectroscopy, zetapotential measurements, contact angle measurements, and atomic force microscopy. The amount of functional groups is determined by pH potentiometric titration as well as the ninhydrin test and is in the range of 25 pmolcm⁻². Adsorption of bovine serum albumine (BSA) and fibrinogen on the cellulose film and the cellulose lysine carbamate surface is studied at different pH values by quartz crystal microbalance with dissipation monitoring (QCM-D). The adsorption of BSA is significantly reduced by modification with lysine. At physiological pH value (7.4) the adsorption of fibrinogen is even six times lower (1.0 mgm⁻²) compared to the pure cellulose surface. Thus, cellulose thin films with lysine moieties are promising candidates for hemo-compatible antifouling surfaces in the field of blood contacting devices.

Original language	English
Pages (from-to)	537-547
Number of pages	11
Journal	Cellulose
Volume	25
Issue number	1
DOIs	https://doi.org/10.1007/s10570-017-1538-9
Publication status	Published - 1 Jan 2018
Externally published	Yes

Keywords

BSA
Cellulose lysine carbamate
Fibrinogen
Protein adsorption
Thin films

ASJC Scopus subject areas

Polymers and Plastics

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10.1007/s10570-017-1538-9

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title = "Modification of cellulose thin films with lysine moieties: a promising approach to achieve antifouling performance",

abstract = "Thin films of trimethylsilyl cellulose are obtained by spin coating and regenerated to cellulose. The surface is activated with N,N′-carbonyldiimidazole and aminolysis with lysine is carried out in aqueous solution to yield a cellulose lysine carbamate film. The surface is analytically characterized by ATR-IR spectroscopy, zetapotential measurements, contact angle measurements, and atomic force microscopy. The amount of functional groups is determined by pH potentiometric titration as well as the ninhydrin test and is in the range of 25 pmolcm−2. Adsorption of bovine serum albumine (BSA) and fibrinogen on the cellulose film and the cellulose lysine carbamate surface is studied at different pH values by quartz crystal microbalance with dissipation monitoring (QCM-D). The adsorption of BSA is significantly reduced by modification with lysine. At physiological pH value (7.4) the adsorption of fibrinogen is even six times lower (1.0 mgm−2) compared to the pure cellulose surface. Thus, cellulose thin films with lysine moieties are promising candidates for hemo-compatible antifouling surfaces in the field of blood contacting devices.",

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author = "Thomas Elschner and Matej Bra{\v c}i{\v c} and Tamilselvan Mohan and Rupert Kargl and {Stana Kleinschek}, Karin",

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doi = "10.1007/s10570-017-1538-9",

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T2 - a promising approach to achieve antifouling performance

AU - Elschner, Thomas

AU - Bračič, Matej

AU - Mohan, Tamilselvan

AU - Kargl, Rupert

AU - Stana Kleinschek, Karin

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Thin films of trimethylsilyl cellulose are obtained by spin coating and regenerated to cellulose. The surface is activated with N,N′-carbonyldiimidazole and aminolysis with lysine is carried out in aqueous solution to yield a cellulose lysine carbamate film. The surface is analytically characterized by ATR-IR spectroscopy, zetapotential measurements, contact angle measurements, and atomic force microscopy. The amount of functional groups is determined by pH potentiometric titration as well as the ninhydrin test and is in the range of 25 pmolcm−2. Adsorption of bovine serum albumine (BSA) and fibrinogen on the cellulose film and the cellulose lysine carbamate surface is studied at different pH values by quartz crystal microbalance with dissipation monitoring (QCM-D). The adsorption of BSA is significantly reduced by modification with lysine. At physiological pH value (7.4) the adsorption of fibrinogen is even six times lower (1.0 mgm−2) compared to the pure cellulose surface. Thus, cellulose thin films with lysine moieties are promising candidates for hemo-compatible antifouling surfaces in the field of blood contacting devices.

AB - Thin films of trimethylsilyl cellulose are obtained by spin coating and regenerated to cellulose. The surface is activated with N,N′-carbonyldiimidazole and aminolysis with lysine is carried out in aqueous solution to yield a cellulose lysine carbamate film. The surface is analytically characterized by ATR-IR spectroscopy, zetapotential measurements, contact angle measurements, and atomic force microscopy. The amount of functional groups is determined by pH potentiometric titration as well as the ninhydrin test and is in the range of 25 pmolcm−2. Adsorption of bovine serum albumine (BSA) and fibrinogen on the cellulose film and the cellulose lysine carbamate surface is studied at different pH values by quartz crystal microbalance with dissipation monitoring (QCM-D). The adsorption of BSA is significantly reduced by modification with lysine. At physiological pH value (7.4) the adsorption of fibrinogen is even six times lower (1.0 mgm−2) compared to the pure cellulose surface. Thus, cellulose thin films with lysine moieties are promising candidates for hemo-compatible antifouling surfaces in the field of blood contacting devices.

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KW - Cellulose lysine carbamate

KW - Fibrinogen

KW - Protein adsorption

KW - Thin films

KW - Cellulose lysine carbamate

KW - protein adsorption

KW - fibrinogen

KW - BSA

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ER -

Modification of cellulose thin films with lysine moieties: a promising approach to achieve antifouling performance

Abstract

Keywords

ASJC Scopus subject areas

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