Membrane chromatography for the purification of laccase from the supernatant of Pleurotus sapidus

Mayuratheepan Puthirasigamany, Monika Wirges, Tim Zeiner*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Extracellular laccase from the fermentation broth of Pleurotus sapidus was purified using membrane chromatography. Five laccase iso-enzymes produced in submerged cultures were purified; the enzymes had isoelectric points (p. I) ranging from 3.3 to 4.7 and a calculated molecular weight of 57.4. kDa. The fermentation broth had a low product titre of 80 μg/l. Hydrophobic interaction (HIC) and ion exchange (IEX) membranes chromatography modules were used in an ÄKTApurifier 100 liquid chromatography system for the dynamic experiments. The pre-treatment of the fermentation broth was not required. The enzyme activity was retained, and enrichment up to 15-fold was observed via activity assays and SDS-Page analysis.

Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalBiochemical Engineering Journal
Volume70
DOIs
Publication statusPublished - 15 Jan 2013

Keywords

  • Downstream processing
  • Laccase
  • Membrane adsorption
  • Membrane chromatography
  • Pleurotus sapidus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

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