Membrane chromatography for the purification of laccase from the supernatant of Pleurotus sapidus

Mayuratheepan Puthirasigamany, Monika Wirges, Tim Zeiner

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Extracellular laccase from the fermentation broth of Pleurotus sapidus was purified using membrane chromatography. Five laccase iso-enzymes produced in submerged cultures were purified; the enzymes had isoelectric points (p. I) ranging from 3.3 to 4.7 and a calculated molecular weight of 57.4. kDa. The fermentation broth had a low product titre of 80 μg/l. Hydrophobic interaction (HIC) and ion exchange (IEX) membranes chromatography modules were used in an ÄKTApurifier 100 liquid chromatography system for the dynamic experiments. The pre-treatment of the fermentation broth was not required. The enzyme activity was retained, and enrichment up to 15-fold was observed via activity assays and SDS-Page analysis.

Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalBiochemical Engineering Journal
Volume70
DOIs
Publication statusPublished - 15 Jan 2013

Fingerprint

Pleurotus
Laccase
Chromatography
Fermentation
Purification
Membranes
Enzymes
Ion exchange membranes
Liquid chromatography
Ion Exchange Chromatography
Isoelectric Point
Enzyme activity
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
Assays
Molecular Weight
Molecular weight
Experiments

Keywords

  • Downstream processing
  • Laccase
  • Membrane adsorption
  • Membrane chromatography
  • Pleurotus sapidus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

Cite this

Membrane chromatography for the purification of laccase from the supernatant of Pleurotus sapidus. / Puthirasigamany, Mayuratheepan; Wirges, Monika; Zeiner, Tim.

In: Biochemical Engineering Journal, Vol. 70, 15.01.2013, p. 180-187.

Research output: Contribution to journalArticleResearchpeer-review

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