Melatonin minimizes the impact of oxidative stress induced by hydrogen peroxide in Saccharomyces and Non-conventional yeast

Jennifer Vázquez, Karlheinz Grillitsch, Günther Daum, Albert Mas, María Jesús Torija, Gemma Beltran

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Melatonin (N-acetyl-5-methoxytryptamine) is synthesized from tryptophan by Saccharomyces cerevisiae and non-conventional yeast species. Antioxidant properties have been suggested as a possible role of melatonin in a S. cerevisiae wine strain. However, the possible antioxidant melatonin effect on non-Saccharomyces species and other strains of S. cerevisiae must be evaluated. The aim of this study was to determine the antioxidant capacity of melatonin in eight S. cerevisiae strains and four non-conventional yeasts (Torulaspora delbrueckii, Metschnikowia pulcherrima, Starmerella bacillaris, and Hanseniaspora uvarum). Therefore, the ROS formation, lipid peroxidation, catalase activity, fatty acid composition, and peroxisome proliferation were investigated. The results showed that the presence of melatonin increases peroxisome accumulation and slightly increases the catalase activity. When cells grown in the presence of melatonin were exposed to oxidative stress induced by H2O2, lower ROS accumulation and lipid peroxidation were observed in all tested strains. Therefore, the increased catalase activity that was a consequence of oxidative stress was lower in the presence of melatonin. Moreover, the presence of MEL modulates cell FA composition, increasing oleic and palmitoleic acids and leading to higher UFA/SFA ratios, which have been previously related to a higher tolerance to H2O2. These findings demonstrate that melatonin can act as an antioxidant compound in both S. cerevisiae and non-Saccharomyces yeasts.

Original languageEnglish
Article number1933
JournalFrontiers in Microbiology
Volume9
Issue numberAUG
DOIs
Publication statusPublished - 20 Aug 2018

Fingerprint

Saccharomyces
Melatonin
Hydrogen Peroxide
Oxidative Stress
Yeasts
Saccharomyces cerevisiae
Antioxidants
Catalase
Peroxisomes
Lipid Peroxidation
Torulaspora
Metschnikowia
Hanseniaspora
5-Methoxytryptamine
Oleic Acids
Wine
Tryptophan
Fatty Acids

Keywords

  • Catalase
  • Hanseniaspora uvarum
  • Metschnikowia pulcherrima
  • Peroxisomes
  • ROS
  • Starmerella bacillaris
  • TBARS
  • Torulaspora delbrueckii

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Melatonin minimizes the impact of oxidative stress induced by hydrogen peroxide in Saccharomyces and Non-conventional yeast. / Vázquez, Jennifer; Grillitsch, Karlheinz; Daum, Günther; Mas, Albert; Torija, María Jesús; Beltran, Gemma.

In: Frontiers in Microbiology , Vol. 9, No. AUG, 1933, 20.08.2018.

Research output: Contribution to journalArticleResearchpeer-review

Vázquez, Jennifer ; Grillitsch, Karlheinz ; Daum, Günther ; Mas, Albert ; Torija, María Jesús ; Beltran, Gemma. / Melatonin minimizes the impact of oxidative stress induced by hydrogen peroxide in Saccharomyces and Non-conventional yeast. In: Frontiers in Microbiology . 2018 ; Vol. 9, No. AUG.
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