Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine

Sarah Waldie, Tania K Lind, Kathryn Browning, Martine Mouline, Michael Haertlein, Trevor Forsyth, Alessandra Luchini, Gernot Strohmeier, Harald Pichler, Selma Maric, Marité Cárdenas

Research output: Research - peer-reviewArticle

Abstract

Cholesterol is an essential component of mammalian membranes that is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid ordered phases with an increased thickness and configurational order as compared to liquid disordered phases. For saturated lipid membranes, cholesterol molecules localise close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote the relocation of cholesterol towards the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the localisation of cholesterol within supported lipid bilayers composed of a natural mixture of phosphatidylcholine (PC). The lipids were produced in a genetically
modified strain of E. coli and grown under specific deuterated conditions to give an overall neutron scattering length density (or level of deuteration) of the lipids matching that of D2O. Besides the traditional solvent contrast variation approach, both hydrogenated and per-deuterated cholesterol were used for additional contrast. This approach shows that cholesterol is located closer
to the lipid head group - tail interface in this natural PC extract rather than in the centre of the core of the bilayer as for very thin or polyunsaturated membranes. Moreover, cholesterol preferentially enriches the outer leaflet, possibly due to an asymmetric leaflet distribution in the vesicles used for the supported lipid bilayer formation. Thus, here we report the localisation of cholesterol is studied in bilayers composed of a deuterated, natural phosphatidylcholine extract and a combination of hydrogenated and perdeuterated cholesterol.
LanguageEnglish
Pages472-479
Number of pages8
JournalLangmuir
Volume34
Issue number1
DOIs
StatusE-pub ahead of print - 27 Dec 2017

Fingerprint

Lipid bilayers
Cholesterol
cholesterol
Lipid Bilayers
Phosphatidylcholines
lipids
Lipids
Neutrons
membranes
liquid phases
Neutron reflection
Membranes
relocation
Relocation
Liquids
Membrane Lipids
Neutron scattering
Escherichia coli
neutron scattering
neutrons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)

Cooperations

  • BioTechMed-Graz
  • NAWI Graz

Cite this

Waldie, S., Lind, T. K., Browning, K., Mouline, M., Haertlein, M., Forsyth, T., ... Cárdenas, M. (2017). Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine. Langmuir, 34(1), 472-479. DOI: 10.1021/acs.langmuir.7b02716

Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine. / Waldie, Sarah; Lind, Tania K; Browning, Kathryn; Mouline, Martine; Haertlein, Michael ; Forsyth, Trevor; Luchini, Alessandra; Strohmeier, Gernot; Pichler, Harald; Maric, Selma; Cárdenas, Marité.

In: Langmuir, Vol. 34, No. 1, 27.12.2017, p. 472-479.

Research output: Research - peer-reviewArticle

Waldie, S, Lind, TK, Browning, K, Mouline, M, Haertlein, M, Forsyth, T, Luchini, A, Strohmeier, G, Pichler, H, Maric, S & Cárdenas, M 2017, 'Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine' Langmuir, vol 34, no. 1, pp. 472-479. DOI: 10.1021/acs.langmuir.7b02716
Waldie S, Lind TK, Browning K, Mouline M, Haertlein M, Forsyth T et al. Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine. Langmuir. 2017 Dec 27;34(1):472-479. Available from, DOI: 10.1021/acs.langmuir.7b02716
Waldie, Sarah ; Lind, Tania K ; Browning, Kathryn ; Mouline, Martine ; Haertlein, Michael ; Forsyth, Trevor ; Luchini, Alessandra ; Strohmeier, Gernot ; Pichler, Harald ; Maric, Selma ; Cárdenas, Marité. / Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine. In: Langmuir. 2017 ; Vol. 34, No. 1. pp. 472-479
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