LC method for the direct and simultaneous determination of four major furan derivatives in coffee grounds and brews

Abdullatif Albouchi, Michael Murkovic

Research output: Contribution to journalArticle

Abstract

The simultaneous quantification of two potential genotoxic hydroxymethyl furan
derivatives in coffee (furfuryl alcohol and 5-hydroxymethylfurfural) alongside their carboxylic acid derivatives (2-furoic acid and 5-hydroxymethyl furoic acid, respectively) was carried out. Their extraction from ground roasted coffee using sonication, simple shaking or heat-assisted shaking lead to similar results. A minimum of 97.3% of the four furan derivatives were extracted during the first extraction cycle using water, whereas methanol showed considerably lower extraction efficiency. A simple high-performance liquid chromatography method coupled with diode array detection was developed for the simultaneous determination and was applied to roasted coffee extracts or brews. No sample pre-treatment except for centrifugation was needed.
The diode array detector was used to assess the purity of the peaks of interest in analyzed samples against authentic standards. The linearity according to Mandel, accuracy (recovery ≥ 89.9%) and precision (inter- and intraday relative standard deviation ≤ 4.5%) were checked. The values for the limit of detection and quantification ranged within 0.11–0.76 and 0.35–2.55μg/mL, respectively. Filtered and espresso brews were analyzed for the four furan derivatives where furfuryl alcohol showed double the concentration of 5-hydroxymethylfurfural and about ten times the concentrations of 2-furoic acid or 5-hydroxymethyl furoic acid.
Original languageEnglish
Pages (from-to)1695-1701
Number of pages7
JournalJournal of separation science
Volume42
Issue number9
DOIs
Publication statusPublished - 2019

Keywords

  • coffee
  • extraction solvents
  • extraction techniques
  • furan derivatives
  • high-performance liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Fields of Expertise

  • Human- & Biotechnology

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