Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

Katharina Schmölzer, Melanie Weingarten, Kai Baldenius, Bernd Nidetzky

Research output: Contribution to journalArticleResearchpeer-review


Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase. Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.

Original languageEnglish
Pages (from-to)5661-5665
Number of pages5
JournalOrganic and Biomolecular Chemistry
Issue number23
Publication statusPublished - 1 Jan 2019


ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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