Intensification of Aqueous Two-phase Extraction for Protein Purification

Christoph Brandenbusch, Tim Zeiner*, Juliane Merz

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Within today's downstream processing of therapeutic proteins, there is an imminent need for alternatives to chromatographic separations, such as aqueous two-phase extraction. Challenges in the development and performance of aqueous two-phase extraction include a number of experiments to define appropriate process windows as well as enable multistage extraction. To reduce experimental effort, a hybrid thermodynamic model is presented in this contribution which allows the extraction system to be optimized with a smaller number of experiments. Moreover, multistage applications of aqueous two-phase extraction are presented to purify enzymes. To purify the investigated enzymes, two different apparatuses were used: namely, a mixer-settler unit and a centrifugal partition chromatograph.

Original languageEnglish
Title of host publicationCatalyst-free Organic Synthesis
PublisherRoyal Society of Chemistry
Number of pages21
Publication statusPublished - 1 Jan 2018

Publication series

NameRSC Green Chemistry
ISSN (Print)1757-7039
ISSN (Electronic)1757-7047

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Chemistry
  • Pollution
  • Management, Monitoring, Policy and Law


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