Intensification of Aqueous Two-phase Extraction for Protein Purification

Christoph Brandenbusch, Tim Zeiner, Juliane Merz

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Abstract

Within today's downstream processing of therapeutic proteins, there is an imminent need for alternatives to chromatographic separations, such as aqueous two-phase extraction. Challenges in the development and performance of aqueous two-phase extraction include a number of experiments to define appropriate process windows as well as enable multistage extraction. To reduce experimental effort, a hybrid thermodynamic model is presented in this contribution which allows the extraction system to be optimized with a smaller number of experiments. Moreover, multistage applications of aqueous two-phase extraction are presented to purify enzymes. To purify the investigated enzymes, two different apparatuses were used: namely, a mixer-settler unit and a centrifugal partition chromatograph.

Original languageEnglish
Title of host publicationCatalyst-free Organic Synthesis
PublisherRoyal Society of Chemistry
Chapter16
Pages344-364
Number of pages21
Edition55
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameRSC Green Chemistry
Number55
Volume2018-January
ISSN (Print)1757-7039
ISSN (Electronic)1757-7047

Fingerprint

Purification
purification
Proteins
protein
Enzymes
Thermodynamics
enzyme
thermodynamics
experiment
Experiments
Therapeutics
Processing

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Chemistry
  • Pollution
  • Management, Monitoring, Policy and Law

Cite this

Brandenbusch, C., Zeiner, T., & Merz, J. (2018). Intensification of Aqueous Two-phase Extraction for Protein Purification. In Catalyst-free Organic Synthesis (55 ed., pp. 344-364). (RSC Green Chemistry; Vol. 2018-January, No. 55). Royal Society of Chemistry. https://doi.org/10.1039/9781788010320-00344

Intensification of Aqueous Two-phase Extraction for Protein Purification. / Brandenbusch, Christoph; Zeiner, Tim; Merz, Juliane.

Catalyst-free Organic Synthesis. 55. ed. Royal Society of Chemistry, 2018. p. 344-364 (RSC Green Chemistry; Vol. 2018-January, No. 55).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Brandenbusch, C, Zeiner, T & Merz, J 2018, Intensification of Aqueous Two-phase Extraction for Protein Purification. in Catalyst-free Organic Synthesis. 55 edn, RSC Green Chemistry, no. 55, vol. 2018-January, Royal Society of Chemistry, pp. 344-364. https://doi.org/10.1039/9781788010320-00344
Brandenbusch C, Zeiner T, Merz J. Intensification of Aqueous Two-phase Extraction for Protein Purification. In Catalyst-free Organic Synthesis. 55 ed. Royal Society of Chemistry. 2018. p. 344-364. (RSC Green Chemistry; 55). https://doi.org/10.1039/9781788010320-00344
Brandenbusch, Christoph ; Zeiner, Tim ; Merz, Juliane. / Intensification of Aqueous Two-phase Extraction for Protein Purification. Catalyst-free Organic Synthesis. 55. ed. Royal Society of Chemistry, 2018. pp. 344-364 (RSC Green Chemistry; 55).
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