Integration and application of optical chemical sensors in microbioreactors

Pia Gruber, Marco P C Marques, Nicolas Szita, Torsten Mayr

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The quantification of key variables such as oxygen, pH, carbon dioxide, glucose, and temperature provides essential information for biological and biotechnological applications and their development. Microfluidic devices offer an opportunity to accelerate research and development in these areas due to their small scale, and the fine control over the microenvironment, provided that these key variables can be measured. Optical sensors are well-suited for this task. They offer non-invasive and non-destructive monitoring of the mentioned variables, and the establishment of time-course profiles without the need for sampling from the microfluidic devices. They can also be implemented in larger systems, facilitating cross-scale comparison of analytical data. This tutorial review presents an overview of the optical sensors and their technology, with a view to support current and potential new users in microfluidics and biotechnology in the implementation of such sensors. It introduces the benefits and challenges of sensor integration, including, their application for microbioreactors. Sensor formats, integration methods, device bonding options, and monitoring options are explained. Luminescent sensors for oxygen, pH, carbon dioxide, glucose and temperature are showcased. Areas where further development is needed are highlighted with the intent to guide future development efforts towards analytes for which reliable, stable, or easily integrated detection methods are not yet available.

Original languageEnglish
Pages (from-to)2693-2712
Number of pages20
JournalChip
Volume17
Issue number16
DOIs
Publication statusPublished - 8 Aug 2017

Fingerprint

Lab-On-A-Chip Devices
Carbon Dioxide
Oxygen
Glucose
Temperature
Microfluidics
Biotechnology
Technology
Equipment and Supplies
Research

Keywords

  • Journal Article

Fields of Expertise

  • Human- & Biotechnology

Cite this

Integration and application of optical chemical sensors in microbioreactors. / Gruber, Pia; Marques, Marco P C; Szita, Nicolas; Mayr, Torsten.

In: Chip, Vol. 17, No. 16, 08.08.2017, p. 2693-2712.

Research output: Contribution to journalArticleResearchpeer-review

Gruber, Pia ; Marques, Marco P C ; Szita, Nicolas ; Mayr, Torsten. / Integration and application of optical chemical sensors in microbioreactors. In: Chip. 2017 ; Vol. 17, No. 16. pp. 2693-2712.
@article{4216d9a4b1fc4faab3dfd2b4adb8e394,
title = "Integration and application of optical chemical sensors in microbioreactors",
abstract = "The quantification of key variables such as oxygen, pH, carbon dioxide, glucose, and temperature provides essential information for biological and biotechnological applications and their development. Microfluidic devices offer an opportunity to accelerate research and development in these areas due to their small scale, and the fine control over the microenvironment, provided that these key variables can be measured. Optical sensors are well-suited for this task. They offer non-invasive and non-destructive monitoring of the mentioned variables, and the establishment of time-course profiles without the need for sampling from the microfluidic devices. They can also be implemented in larger systems, facilitating cross-scale comparison of analytical data. This tutorial review presents an overview of the optical sensors and their technology, with a view to support current and potential new users in microfluidics and biotechnology in the implementation of such sensors. It introduces the benefits and challenges of sensor integration, including, their application for microbioreactors. Sensor formats, integration methods, device bonding options, and monitoring options are explained. Luminescent sensors for oxygen, pH, carbon dioxide, glucose and temperature are showcased. Areas where further development is needed are highlighted with the intent to guide future development efforts towards analytes for which reliable, stable, or easily integrated detection methods are not yet available.",
keywords = "Journal Article",
author = "Pia Gruber and Marques, {Marco P C} and Nicolas Szita and Torsten Mayr",
year = "2017",
month = "8",
day = "8",
doi = "10.1039/c7lc00538e",
language = "English",
volume = "17",
pages = "2693--2712",
journal = "Chip",
issn = "0170-6632",
publisher = "Chip Communications GmbH",
number = "16",

}

TY - JOUR

T1 - Integration and application of optical chemical sensors in microbioreactors

AU - Gruber, Pia

AU - Marques, Marco P C

AU - Szita, Nicolas

AU - Mayr, Torsten

PY - 2017/8/8

Y1 - 2017/8/8

N2 - The quantification of key variables such as oxygen, pH, carbon dioxide, glucose, and temperature provides essential information for biological and biotechnological applications and their development. Microfluidic devices offer an opportunity to accelerate research and development in these areas due to their small scale, and the fine control over the microenvironment, provided that these key variables can be measured. Optical sensors are well-suited for this task. They offer non-invasive and non-destructive monitoring of the mentioned variables, and the establishment of time-course profiles without the need for sampling from the microfluidic devices. They can also be implemented in larger systems, facilitating cross-scale comparison of analytical data. This tutorial review presents an overview of the optical sensors and their technology, with a view to support current and potential new users in microfluidics and biotechnology in the implementation of such sensors. It introduces the benefits and challenges of sensor integration, including, their application for microbioreactors. Sensor formats, integration methods, device bonding options, and monitoring options are explained. Luminescent sensors for oxygen, pH, carbon dioxide, glucose and temperature are showcased. Areas where further development is needed are highlighted with the intent to guide future development efforts towards analytes for which reliable, stable, or easily integrated detection methods are not yet available.

AB - The quantification of key variables such as oxygen, pH, carbon dioxide, glucose, and temperature provides essential information for biological and biotechnological applications and their development. Microfluidic devices offer an opportunity to accelerate research and development in these areas due to their small scale, and the fine control over the microenvironment, provided that these key variables can be measured. Optical sensors are well-suited for this task. They offer non-invasive and non-destructive monitoring of the mentioned variables, and the establishment of time-course profiles without the need for sampling from the microfluidic devices. They can also be implemented in larger systems, facilitating cross-scale comparison of analytical data. This tutorial review presents an overview of the optical sensors and their technology, with a view to support current and potential new users in microfluidics and biotechnology in the implementation of such sensors. It introduces the benefits and challenges of sensor integration, including, their application for microbioreactors. Sensor formats, integration methods, device bonding options, and monitoring options are explained. Luminescent sensors for oxygen, pH, carbon dioxide, glucose and temperature are showcased. Areas where further development is needed are highlighted with the intent to guide future development efforts towards analytes for which reliable, stable, or easily integrated detection methods are not yet available.

KW - Journal Article

U2 - 10.1039/c7lc00538e

DO - 10.1039/c7lc00538e

M3 - Article

VL - 17

SP - 2693

EP - 2712

JO - Chip

JF - Chip

SN - 0170-6632

IS - 16

ER -