Influence of the relative humidity on aminosilane molecular grafting properties

Fabio Lisi, Davide Carta, Laura Villanova, Irene Poli, Dario Buso, Stefano Costacurta, Anita J. Hill, Paolo Falcaro*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Functional coatings with amino groups are used in a wide range of biochemistry-related applications. A technological platform that takes advantage of the affinity between amino-functionalized coatings and biomolecules is the DNA microarray methodology. Reliability and reproducibility of the microarray data strongly depend on the quality of the substrate; therefore, a proper awareness of how the storage conditions affect the amino-functionalized coatings is necessary. In this work we have studied the influence of different relative humidity levels on aminomethyl- silane coatings prepared via sol-gel methodology. Drops of a buffer solution containing a luminescent dye have been deposited (or spotted) on the coating; the dye molecules react with the amino-groups and leave circular luminescent marks (spots) on the substrate surface. Shape and luminescence of the spots, as well as background signals, have been monitored using a microarray laser scanner. With the proposed protocol we have measured the changes of these variables due to different storage conditions. FT-IR measurements have been performed to investigate the related chemistry changes.

Original languageEnglish
Pages (from-to)246-253
Number of pages8
JournalJournal of sol gel science and technology
Volume60
Issue number3
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Keywords

  • Aminopropyltriethoxysilane
  • Fluorescein isothiocyanate
  • Grafting
  • Microarray
  • Sol-gel
  • Storage conditions

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Biomaterials
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

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