Influence of the N-terminal segment and the PHY-tongue element on light-regulation in bacteriophytochromes

Geoffrey Gourinchas, Uršula Vide, Andreas Winkler

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Photoreceptors enable the integration of ambient light stimuli to trigger lifestyle adaptations via modulation of central metabolite levels involved in diverse regulatory processes. Red light-sensing bacteriophytochromes are attractive targets for the development of innovative optogenetic tools because of their natural modularity of coupling with diverse functionalities and the natural availability of the light-absorbing biliverdin chromophore in animal tissues. However, a rational design of such tools is complicated by the poor understanding of molecular mechanisms of light signal transduction over long distances-from the site of photon absorption to the active site of downstream enzymatic effectors. Here we show how swapping structural elements between two bacteriophytochrome homologs provides additional insight into light signal integration and effector regulation, involving a fine-tuned interplay of important structural elements of the sensor, as well as the sensor-effector linker. Facilitated by the availability of structural information of inhibited and activated full-length structures of one of the two homologs (Idiomarina species A28L phytochrome-activated diguanylyl cyclase (IsPadC)) and characteristic differences in photoresponses of the two homologs, we identify an important cross-talk between the N-terminal segment, containing the covalent attachment site of the chromophore, and the PHY-tongue region. Moreover, we highlight how these elements influence the dynamic range of photoactivation and how activation can be improved to light/dark ratios of ∼800-fold by reducing basal dark-state activities at the same time as increasing conversion in the light state. This will enable future optimization of optogenetic tools aiming at a direct allosteric regulation of enzymatic effectors.

Original languageEnglish
Pages (from-to)4498-4510
Number of pages13
JournalThe Journal of Biological Chemistry
Volume294
Issue number12
DOIs
Publication statusPublished - 22 Mar 2019

Fingerprint

Tongue
Light
Optogenetics
Chromophores
Availability
Alteromonadaceae
Biliverdine
Phytochrome
Signal transduction
Allosteric Regulation
Light Signal Transduction
Sensors
Metabolites
Photons
Animals
Chemical activation
Modulation
Life Style
Catalytic Domain
Tissue

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Influence of the N-terminal segment and the PHY-tongue element on light-regulation in bacteriophytochromes. / Gourinchas, Geoffrey; Vide, Uršula; Winkler, Andreas.

In: The Journal of Biological Chemistry, Vol. 294, No. 12, 22.03.2019, p. 4498-4510.

Research output: Contribution to journalArticleResearchpeer-review

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