Abstract
(Figure Presented) Controlling the context: A novel strategy for the in vitro expression and immediate post-translational membrane insertion of complex membrane proteins into an artificial membrane system is described. In this way problems are circumvented that arise when complex membrane proteins are overexpressed, detergent-solubilized, and subsequently reconstituted into suitable artificial membrane systems for biophysical characterization. GPCR = G-protein coupled receptor.
Original language | English |
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Pages (from-to) | 605-608 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 46 |
Issue number | 4 |
DOIs | |
Publication status | Published - 31 Jan 2007 |
Externally published | Yes |
Keywords
- Bioorganic chemistry
- Fluorescence spectroscopy
- In vitro synthesis
- Membrane proteins
- Membranes
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)