Incorporation of in vitro synthesized GPCR into a tethered artificial lipid membrane system

Rudolf Robelek, Eva S. Lemker, Birgit Wiltschi, Vinzenz Kirste, Renate Naumann, Dieter Oesterhelt, Eva Kathrin Sinner*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

(Figure Presented) Controlling the context: A novel strategy for the in vitro expression and immediate post-translational membrane insertion of complex membrane proteins into an artificial membrane system is described. In this way problems are circumvented that arise when complex membrane proteins are overexpressed, detergent-solubilized, and subsequently reconstituted into suitable artificial membrane systems for biophysical characterization. GPCR = G-protein coupled receptor.

Original languageEnglish
Pages (from-to)605-608
Number of pages4
JournalAngewandte Chemie - International Edition
Volume46
Issue number4
DOIs
Publication statusPublished - 31 Jan 2007
Externally publishedYes

Keywords

  • Bioorganic chemistry
  • Fluorescence spectroscopy
  • In vitro synthesis
  • Membrane proteins
  • Membranes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Incorporation of in vitro synthesized GPCR into a tethered artificial lipid membrane system'. Together they form a unique fingerprint.

Cite this