Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Hana Raschmanová, Astrid Weninger, Anton Glieder, Karin Kovar, Thomas Vogl

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

Original languageEnglish
Pages (from-to)641-665
Number of pages25
JournalBiotechnology Advances
Volume36
Issue number3
DOIs
Publication statusPublished - 1 May 2018

Keywords

  • Candida albicans/glabrata
  • CRISPR-Cas
  • Expression optimization
  • Metabolic engineering
  • Pichia pastoris
  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Synthetic biology
  • Yarrowia lipolytica
  • Yeasts

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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