Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols

Robert Kourist, Sajja Hari Krishna, Jesal S Patel, Flash Bartnek, Timothy S Hitchman, David P Weiner, Uwe T Bornscheuer

Research output: Contribution to journalArticleResearchpeer-review

Abstract

35 metagenome-derived esterases bearing a GGG(A)X motif were screened for activity and enantioselectivity in the hydrolysis of a range of tertiary alcohol acetates. Most of the active esterases showed little or no enantioselectivity in the hydrolysis of the terpinyl acetate, linalyl acetate and 3-methylpent-1-yn-3-yl acetate. However, one esterase showed excellent enantioselectivity (E > 100) in the kinetic resolution of 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate as confirmed by a preparative scale reaction.

Original languageEnglish
Pages (from-to)3310-3313
Number of pages4
JournalOrganic & biomolecular chemistry
Volume5
Issue number20
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Keywords

  • Acetates
  • Esterases
  • Gene Library
  • Genome
  • Kinetics
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fields of Expertise

  • Human- & Biotechnology

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