High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low-Density Lipoprotein Nanoparticles

Bernhard Lehofer, Maksym Golub, Karin Kornmueller, Manfred Kriechbaum, Nicolas Martinez, Gergely Nagy, Joachim Kohlbrecher, Heinz Amenitsch, Judith Peters, Ruth Prassl

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Low-density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X-ray and neutron small-angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride-rich and oxidized forms of LDL. Due to the different scattering contrasts for X-rays and neutrons, information on the effects of HHP on the internal structure determined by lipid rearrangements and changes in particle shape becomes accessible. Independent pressure and temperature variations provoke a phase transition in the lipid core domain. With increasing pressure an interrelated anisotropic deformation and flattening of the particle are induced. All LDL nanoparticles maintain their structural integrity even at 3000 bar and show a reversible response toward pressure variations. The present work depicts the complementarity of pressure and temperature as independent thermodynamic parameters and introduces HHP as a tool to study molecular assembling and interaction processes in distinct lipoprotein particles in a nondestructive manner.

Original languageEnglish
Article number1800149
JournalParticle and Particle Systems Characterization
Volume35
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018

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Keywords

  • high hydrostatic pressure
  • lipid phase transition
  • Low-density lipoprotein
  • nanoparticle structure
  • small-angle scattering techniques

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Fields of Expertise

  • Human- & Biotechnology

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