Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1

Pascal Mülner, Elisa Schwarz, Kristin Dietel, Stefanie Herfort, Jennifer Jähne, Peter Lasch, Tomislav Cernava, Gabriele Berg, Joachim Vater*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnology. Lipopeptides produced by such organisms play a major role in their potential to inactivate pathogens. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by Paenibacillus polymyxa strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variability. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains, novel but not yet completely characterized polymyxin species were detected, which possibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins have gained increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC–MS approach using different cultivation media. Production of volatile organic compounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an efficient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of Paenibacilli is the formation of volatile pyrazine derivatives
Original languageEnglish
Article number1485
JournalPathogens
Volume10
Issue number11
DOIs
Publication statusPublished - Nov 2021

Keywords

  • Bioactive peptides
  • GC–MS
  • MALDI-TOF mass spectrometry
  • P. polymyxa
  • Volatiles

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Immunology and Microbiology(all)
  • Molecular Biology
  • Immunology and Allergy

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