Fully automatized high-throughput enzyme library screening using a robotic platform

Mark Dörr; Michael P C Fibinger; Daniel Last; Sandy Schmidt; Javier Santos-Aberturas; Dominique Böttcher; Anke Hummel; Clare Vickers; Moritz Voss; Uwe T. Bornscheuer

doi:10.1002/bit.25925

Fully automatized high-throughput enzyme library screening using a robotic platform

Mark Dörr, Michael P C Fibinger, Daniel Last, Sandy Schmidt, Javier Santos-Aberturas, Dominique Böttcher, Anke Hummel, Clare Vickers, Moritz Voss, Uwe T. Bornscheuer

Research output: Contribution to journal › Article › peer-review

Abstract

A fully automatized robotic platform has been established to facilitate high-throughput screening for protein engineering purposes. This platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings. The performance of this system has been exemplified for four enzyme classes important for biocatalysis such as Baeyer-Villiger monooxygenase, transaminase, dehalogenase and acylase in the high-throughput screening of various mutant libraries. This allowed the identification of novel enzyme variants in a sophisticated and highly reliable manner. Furthermore, the detailed optimization protocols should enable other researchers to adapt and improve their methods. Biotechnol. Bioeng. 2016;113: 1421-1432. © 2016 Wiley Periodicals, Inc.

Original language	English
Pages (from-to)	1421-32
Number of pages	12
Journal	Biotechnology and Bioengineering
Volume	113
Issue number	7
DOIs	https://doi.org/10.1002/bit.25925
Publication status	Published - Jul 2016
Externally published	Yes

Keywords

Automation, Laboratory
Enzyme Assays
Equipment Design
High-Throughput Screening Assays
Protein Engineering
Robotics
Small Molecule Libraries
Transaminases
Journal Article

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10.1002/bit.25925

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title = "Fully automatized high-throughput enzyme library screening using a robotic platform",

abstract = "A fully automatized robotic platform has been established to facilitate high-throughput screening for protein engineering purposes. This platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings. The performance of this system has been exemplified for four enzyme classes important for biocatalysis such as Baeyer-Villiger monooxygenase, transaminase, dehalogenase and acylase in the high-throughput screening of various mutant libraries. This allowed the identification of novel enzyme variants in a sophisticated and highly reliable manner. Furthermore, the detailed optimization protocols should enable other researchers to adapt and improve their methods. Biotechnol. Bioeng. 2016;113: 1421-1432. {\textcopyright} 2016 Wiley Periodicals, Inc.",

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author = "Mark D{\"o}rr and Fibinger, {Michael P C} and Daniel Last and Sandy Schmidt and Javier Santos-Aberturas and Dominique B{\"o}ttcher and Anke Hummel and Clare Vickers and Moritz Voss and Bornscheuer, {Uwe T.}",

note = "{\textcopyright} 2016 Wiley Periodicals, Inc.",

year = "2016",

month = jul,

doi = "10.1002/bit.25925",

language = "English",

volume = "113",

pages = "1421--32",

journal = "Biotechnology and Bioengineering",

issn = "0006-3592",

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AU - Dörr, Mark

AU - Fibinger, Michael P C

AU - Last, Daniel

AU - Schmidt, Sandy

AU - Santos-Aberturas, Javier

AU - Böttcher, Dominique

AU - Hummel, Anke

AU - Vickers, Clare

AU - Voss, Moritz

AU - Bornscheuer, Uwe T.

PY - 2016/7

Y1 - 2016/7

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AB - A fully automatized robotic platform has been established to facilitate high-throughput screening for protein engineering purposes. This platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings. The performance of this system has been exemplified for four enzyme classes important for biocatalysis such as Baeyer-Villiger monooxygenase, transaminase, dehalogenase and acylase in the high-throughput screening of various mutant libraries. This allowed the identification of novel enzyme variants in a sophisticated and highly reliable manner. Furthermore, the detailed optimization protocols should enable other researchers to adapt and improve their methods. Biotechnol. Bioeng. 2016;113: 1421-1432. © 2016 Wiley Periodicals, Inc.

KW - Automation, Laboratory

KW - Enzyme Assays

KW - Equipment Design

KW - High-Throughput Screening Assays

KW - Protein Engineering

KW - Robotics

KW - Small Molecule Libraries

KW - Transaminases

KW - Journal Article

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DO - 10.1002/bit.25925

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SP - 1421

EP - 1432

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 7

ER -

Fully automatized high-throughput enzyme library screening using a robotic platform

Abstract

Keywords

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