Fully automatized high-throughput enzyme library screening using a robotic platform

Mark Dörr, Michael P C Fibinger, Daniel Last, Sandy Schmidt, Javier Santos-Aberturas, Dominique Böttcher, Anke Hummel, Clare Vickers, Moritz Voss, Uwe T. Bornscheuer

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A fully automatized robotic platform has been established to facilitate high-throughput screening for protein engineering purposes. This platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings. The performance of this system has been exemplified for four enzyme classes important for biocatalysis such as Baeyer-Villiger monooxygenase, transaminase, dehalogenase and acylase in the high-throughput screening of various mutant libraries. This allowed the identification of novel enzyme variants in a sophisticated and highly reliable manner. Furthermore, the detailed optimization protocols should enable other researchers to adapt and improve their methods. Biotechnol. Bioeng. 2016;113: 1421-1432. © 2016 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)1421-32
Number of pages12
JournalBiotechnology and Bioengineering
Volume113
Issue number7
DOIs
Publication statusPublished - Jul 2016
Externally publishedYes

Fingerprint

Robotics
Screening
Enzymes
Throughput
amidase
Biocatalysis
Proteins
Protein Engineering
Protein Stability
Mutant Proteins
Mixed Function Oxygenases
Transaminases
Assays
Research Personnel
Monitoring
Growth

Keywords

  • Automation, Laboratory
  • Enzyme Assays
  • Equipment Design
  • High-Throughput Screening Assays
  • Protein Engineering
  • Robotics
  • Small Molecule Libraries
  • Transaminases
  • Journal Article

Cite this

Dörr, M., Fibinger, M. P. C., Last, D., Schmidt, S., Santos-Aberturas, J., Böttcher, D., ... Bornscheuer, U. T. (2016). Fully automatized high-throughput enzyme library screening using a robotic platform. Biotechnology and Bioengineering, 113(7), 1421-32. https://doi.org/10.1002/bit.25925

Fully automatized high-throughput enzyme library screening using a robotic platform. / Dörr, Mark; Fibinger, Michael P C; Last, Daniel; Schmidt, Sandy; Santos-Aberturas, Javier; Böttcher, Dominique; Hummel, Anke; Vickers, Clare; Voss, Moritz; Bornscheuer, Uwe T.

In: Biotechnology and Bioengineering, Vol. 113, No. 7, 07.2016, p. 1421-32.

Research output: Contribution to journalArticleResearchpeer-review

Dörr, M, Fibinger, MPC, Last, D, Schmidt, S, Santos-Aberturas, J, Böttcher, D, Hummel, A, Vickers, C, Voss, M & Bornscheuer, UT 2016, 'Fully automatized high-throughput enzyme library screening using a robotic platform' Biotechnology and Bioengineering, vol. 113, no. 7, pp. 1421-32. https://doi.org/10.1002/bit.25925
Dörr, Mark ; Fibinger, Michael P C ; Last, Daniel ; Schmidt, Sandy ; Santos-Aberturas, Javier ; Böttcher, Dominique ; Hummel, Anke ; Vickers, Clare ; Voss, Moritz ; Bornscheuer, Uwe T. / Fully automatized high-throughput enzyme library screening using a robotic platform. In: Biotechnology and Bioengineering. 2016 ; Vol. 113, No. 7. pp. 1421-32.
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