Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Steven P. Hanlon, Andrea Camattari, Sandra Abad, Anton Glieder, Beat Wirz, Matthias Kittelmann, Stephan Lütz, Margit Winkler

Research output: Contribution to journalArticle

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

Original languageEnglish
Pages (from-to)6001-6003
Number of pages3
JournalChemical Communications
Volume48
Issue number48
DOIs
Publication statusPublished - 2012

Keywords

  • Enzymes
  • Escherichia coli
  • Flavins
  • Humans
  • Mixed Function Oxygenases
  • Moclobemide
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Experimental
  • Application

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