Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Steven P. Hanlon, Andrea Camattari, Sandra Abad, Anton Glieder, Beat Wirz, Matthias Kittelmann, Stephan Lütz, Margit Winkler

Research output: Contribution to journalArticle

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

LanguageEnglish
Pages6001-6003
Number of pages3
JournalChemical communications
Volume48
Issue number48
DOIs
StatusPublished - 2012

Fingerprint

Moclobemide
Mixed Function Oxygenases
Oxides
Drug Compounding
Escherichia coli
Enzymes
4,6-dinitro-o-cresol

Keywords

  • Enzymes
  • Escherichia coli
  • Flavins
  • Humans
  • Mixed Function Oxygenases
  • Moclobemide
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Experimental
  • Application

Cite this

Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale. / Hanlon, Steven P.; Camattari, Andrea; Abad, Sandra; Glieder, Anton; Wirz, Beat; Kittelmann, Matthias; Lütz, Stephan; Winkler, Margit.

In: Chemical communications, Vol. 48, No. 48, 2012, p. 6001-6003.

Research output: Contribution to journalArticle

Hanlon, Steven P. ; Camattari, Andrea ; Abad, Sandra ; Glieder, Anton ; Wirz, Beat ; Kittelmann, Matthias ; Lütz, Stephan ; Winkler, Margit. / Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale. In: Chemical communications. 2012 ; Vol. 48, No. 48. pp. 6001-6003
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AU - Wirz,Beat

AU - Kittelmann,Matthias

AU - Lütz,Stephan

AU - Winkler,Margit

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KW - Enzymes

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KW - Mixed Function Oxygenases

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KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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