Experimental investigation of laccase purification using aqueous two-phase extraction

Axel Prinz; Tim Zeiner; Thomas Vössing; Ina Schüttmann; Holger Zorn; Andrzej Górak

doi:10.3303/CET1227059

Experimental investigation of laccase purification using aqueous two-phase extraction

Axel Prinz, Tim Zeiner^*, Thomas Vössing, Ina Schüttmann, Holger Zorn, Andrzej Górak

^*Corresponding author for this work

Institute of Chemical Engineering and Environmental Technology (6670)

Research output: Contribution to journal › Article › peer-review

Abstract

In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

Original language	English
Pages (from-to)	349-354
Number of pages	6
Journal	Chemical Engineering Transactions
Volume	27
DOIs	https://doi.org/10.3303/CET1227059
Publication status	Published - 2012

ASJC Scopus subject areas

Chemical Engineering(all)

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AU - Prinz, Axel

AU - Zeiner, Tim

AU - Vössing, Thomas

AU - Schüttmann, Ina

AU - Zorn, Holger

AU - Górak, Andrzej

PY - 2012

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AB - In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

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Experimental investigation of laccase purification using aqueous two-phase extraction

Abstract

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