Evolved Peroxygenase-Aryl Alcohol Oxidase Fusions for Self-Sufficient Oxyfunctionalization Reactions

Patricia Gomez De Santos, Sofia Lazaro, Javier Vinã-Gonzalez, Manh Dat Hoang, Israel Sánchez-Moreno, Anton Glieder, Frank Hollmann, Miguel Alcalde*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Fungal peroxygenases are deemed emergent biocatalysts for selective C-H bond oxyfunctionalization reactions. In this study, we have engineered a functional and stable self-sufficient chimeric peroxygenase-oxidase fusion. The bifunctional biocatalyst carried a laboratory-evolved version of the fungal peroxygenase fused to an evolved fungal aryl-alcohol oxidase that supplies H2O2 in situ. Enzyme fusion libraries with peptide linkers of different sizes and amino acid compositions were designed, while attached leader sequences favored secretion in yeast. The most promising functional enzyme fusions were characterized biochemically and further tested for the synthesis of dextrorphan, a metabolite of the antitussive drug dextromethorphan. This reaction system was optimized to control the aromatic alcohol transformation rate, and therefore the H2O2 supply, to achieve total turnover numbers of 62,000, the highest value reported for the biocatalytic synthesis of dextrorphan to date. Accordingly, our study opens an avenue for the use of peroxygenase-aryl alcohol oxidase fusions in the pharmaceutical and chemical sectors.

Original languageEnglish
Pages (from-to)13524-13534
Number of pages11
JournalACS Catalysis
Volume10
Issue number22
DOIs
Publication statusPublished - 20 Nov 2020

Keywords

  • aryl alcohol oxidase
  • enzyme fusion
  • human drug metabolites
  • in situ HOgeneration
  • unspecific peroxygenase

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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