Evolution of the protein corona of lipid gene vectors as a function of plasma concentration

Giulio Caracciolo, Daniela Pozzi, Anna Laura Capriotti, Chiara Cavaliere, Patrizia Foglia, Heinz Amenitsch, Aldo Lagana

Research output: Contribution to journalArticle

Abstract

The concept that the effective unit of interest in the cell–nanomaterial interaction is the particle and its corona of associated proteins is emerging. Here we investigate the compositional evolution of the protein corona of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) and DOTAP/DNA lipoplexes over a wide range of plasma concentrations (2.5–80%). The composition of the hard corona of lipoplexes is quite stable, but that of CLs does evolve considerably. We show that the protein corona of CLs is made of both low-affinity and competitive-binding proteins whose relative abundance changes with the plasma concentration. This result may have deep biological implications for the application of lipid-based gene vectors both in vitro and in vivo.
Original languageEnglish
Pages (from-to)15048-15053
JournalLangmuir
Volume27
Issue number24
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)

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