Enterobacteriaceae dominate the core microbiome and contribute to the resistome of arugula (Eruca sativa Mill.)

Tomislav Cernava, Armin Erlacher, Jung Soh, Christoph W Sensen, Martin Grube, Gabriele Berg

Research output: Contribution to journalArticleResearchpeer-review

Abstract

BACKGROUND: Arugula is a traditional medicinal plant and popular leafy green today. It is mainly consumed raw in the Western cuisine and known to contain various bioactive secondary metabolites. However, arugula has been also associated with high-profile outbreaks causing severe food-borne human diseases. A multiphasic approach integrating data from metagenomics, amplicon sequencing, and arugula-derived bacterial cultures was employed to understand the specificity of the indigenous microbiome and resistome of the edible plant parts.

RESULTS: Our results indicate that arugula is colonized by a diverse, plant habitat-specific microbiota. The indigenous phyllosphere bacterial community was shown to be dominated by Enterobacteriaceae, which are well-equipped with various antibiotic resistances. Unexpectedly, the prevalence of specific resistance mechanisms targeting therapeutic antibiotics (fluoroquinolone, chloramphenicol, phenicol, macrolide, aminocoumarin) was only surpassed by efflux pump assignments.

CONCLUSIONS: Enterobacteria, being core microbiome members of arugula, have a substantial implication in the overall resistome. Detailed insights into the natural occurrence of antibiotic resistances in arugula-associated microorganisms showed that the plant is a hotspot for distinctive defense mechanisms. The specific functioning of microorganisms in this unusual ecosystem provides a unique model to study antibiotic resistances in an ecological context.

Original languageEnglish
Article number13
Pages (from-to)13
JournalMicrobiome
Volume7
Issue number1
DOIs
Publication statusPublished - 29 Jan 2019

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Microbiota
Enterobacteriaceae
Microbial Drug Resistance
Ecosystem
Aminocoumarins
Metagenomics
Edible Plants
Foodborne Diseases
Fluoroquinolones
Macrolides
Chloramphenicol
Medicinal Plants
Disease Outbreaks
Anti-Bacterial Agents
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Enterobacteriaceae dominate the core microbiome and contribute to the resistome of arugula (Eruca sativa Mill.). / Cernava, Tomislav; Erlacher, Armin; Soh, Jung; Sensen, Christoph W; Grube, Martin; Berg, Gabriele.

In: Microbiome, Vol. 7, No. 1, 13, 29.01.2019, p. 13.

Research output: Contribution to journalArticleResearchpeer-review

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