Effects of resveratrol on the structure and fluidity of lipid bilayers: A membrane biophysical study

A. R. Neves, C. Nunes, H. Amenitsch, S. Reis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Resveratrol is a natural active compound which has been attracting increasing interest due to its several pharmacological effects in cancer prevention, cardiovascular protection and treatment of neurodegenerative disorders and diabetes. The current work investigates how resveratrol affects membrane order and structure, gathering information determined by X-ray scattering analysis, derivative spectrophotometry, fluorescence quenching and fluorescence anisotropy studies. The results indicate that resveratrol is able to be incorporated into DMPC liposome model systems, either fluidizing or stiffening the bilayer, which largely depends on the membrane fluidity state. These findings suggest that the effects of resveratrol resemble cholesterol action on biological membranes, thereby contributing to the regulation of cell membrane structure and fluidity, which may influence the activity of transmembrane proteins and hence control the cell signaling pathways. The regulation of a number of cellular functions, thus may contribute to the pharmacological and therapeutic activities of this compound, explaining its pleiotropic action.

Original languageEnglish
Pages (from-to)2118-2126
Number of pages9
JournalSoft matter
Volume12
Issue number7
DOIs
Publication statusPublished - 2016

Fingerprint

Lipid bilayers
Fluidity
lipids
membranes
Membranes
membrane structures
fluorescence
stiffening
Fluorescence
cholesterol
spectrophotometry
activity (biology)
Cell signaling
Dimyristoylphosphatidylcholine
Biological membranes
Membrane structures
Fluidization
Spectrophotometry
Cell membranes
Medical problems

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Effects of resveratrol on the structure and fluidity of lipid bilayers : A membrane biophysical study. / Neves, A. R.; Nunes, C.; Amenitsch, H.; Reis, S.

In: Soft matter, Vol. 12, No. 7, 2016, p. 2118-2126.

Research output: Contribution to journalArticleResearchpeer-review

@article{84e2e52df5cd4769be4b31e8ff99538e,
title = "Effects of resveratrol on the structure and fluidity of lipid bilayers: A membrane biophysical study",
abstract = "Resveratrol is a natural active compound which has been attracting increasing interest due to its several pharmacological effects in cancer prevention, cardiovascular protection and treatment of neurodegenerative disorders and diabetes. The current work investigates how resveratrol affects membrane order and structure, gathering information determined by X-ray scattering analysis, derivative spectrophotometry, fluorescence quenching and fluorescence anisotropy studies. The results indicate that resveratrol is able to be incorporated into DMPC liposome model systems, either fluidizing or stiffening the bilayer, which largely depends on the membrane fluidity state. These findings suggest that the effects of resveratrol resemble cholesterol action on biological membranes, thereby contributing to the regulation of cell membrane structure and fluidity, which may influence the activity of transmembrane proteins and hence control the cell signaling pathways. The regulation of a number of cellular functions, thus may contribute to the pharmacological and therapeutic activities of this compound, explaining its pleiotropic action.",
author = "Neves, {A. R.} and C. Nunes and H. Amenitsch and S. Reis",
year = "2016",
doi = "10.1039/c5sm02905h",
language = "English",
volume = "12",
pages = "2118--2126",
journal = "Soft matter",
issn = "1744-683X",
publisher = "Royal Society of Chemistry",
number = "7",

}

TY - JOUR

T1 - Effects of resveratrol on the structure and fluidity of lipid bilayers

T2 - A membrane biophysical study

AU - Neves, A. R.

AU - Nunes, C.

AU - Amenitsch, H.

AU - Reis, S.

PY - 2016

Y1 - 2016

N2 - Resveratrol is a natural active compound which has been attracting increasing interest due to its several pharmacological effects in cancer prevention, cardiovascular protection and treatment of neurodegenerative disorders and diabetes. The current work investigates how resveratrol affects membrane order and structure, gathering information determined by X-ray scattering analysis, derivative spectrophotometry, fluorescence quenching and fluorescence anisotropy studies. The results indicate that resveratrol is able to be incorporated into DMPC liposome model systems, either fluidizing or stiffening the bilayer, which largely depends on the membrane fluidity state. These findings suggest that the effects of resveratrol resemble cholesterol action on biological membranes, thereby contributing to the regulation of cell membrane structure and fluidity, which may influence the activity of transmembrane proteins and hence control the cell signaling pathways. The regulation of a number of cellular functions, thus may contribute to the pharmacological and therapeutic activities of this compound, explaining its pleiotropic action.

AB - Resveratrol is a natural active compound which has been attracting increasing interest due to its several pharmacological effects in cancer prevention, cardiovascular protection and treatment of neurodegenerative disorders and diabetes. The current work investigates how resveratrol affects membrane order and structure, gathering information determined by X-ray scattering analysis, derivative spectrophotometry, fluorescence quenching and fluorescence anisotropy studies. The results indicate that resveratrol is able to be incorporated into DMPC liposome model systems, either fluidizing or stiffening the bilayer, which largely depends on the membrane fluidity state. These findings suggest that the effects of resveratrol resemble cholesterol action on biological membranes, thereby contributing to the regulation of cell membrane structure and fluidity, which may influence the activity of transmembrane proteins and hence control the cell signaling pathways. The regulation of a number of cellular functions, thus may contribute to the pharmacological and therapeutic activities of this compound, explaining its pleiotropic action.

UR - http://www.scopus.com/inward/record.url?scp=84958260311&partnerID=8YFLogxK

U2 - 10.1039/c5sm02905h

DO - 10.1039/c5sm02905h

M3 - Article

VL - 12

SP - 2118

EP - 2126

JO - Soft matter

JF - Soft matter

SN - 1744-683X

IS - 7

ER -