Discovery of Polyesterases from Moss-Associated Microorganisms

Christina Andrea Müller, Veronika Perz, Christoph Provasnek, Felice Quartinello, Georg M. Guebitz, Gabriele Berg

Research output: Contribution to journalArticle

Abstract

The growing pollution of the environment with plastic debris is a global threat, which urgently requires biotechnological solutions. Enzymatic recycling not only prevents pollution but also would allow recovery of valuable building blocks. Therefore, we explored the existence of microbial polyesterases in microbial communities associated with the Sphagnum magellanicum moss, a key species within unexploited bog ecosystems. This resulted in the identification of six novel esterases, which were isolated, cloned, and heterologously expressed in Escherichia coli. The esterases were found to hydrolyze the copolyester poly(butylene adipate-co-butylene terephthalate) (PBAT) and the oligomeric model substrate bis[4-(benzoyloxy)butyl] terephthalate (BaBTaBBa). Two promising polyesterase candidates, EstB3 and EstC7, which clustered in family VIII of bacterial lipolytic enzymes, were purified and characterized using the soluble esterase substrate p-nitrophenyl butyrate (Km values of 46.5 and 3.4 µM, temperature optima of 48°C and 50°C, and pH optima of 7.0 and 8.5, respectively). In particular, EstC7 showed outstanding activity and a strong preference for hydrolysis of the aromatic ester bond in PBAT. Our study highlights the potential of plant-associated microbiomes from extreme natural ecosystems as a source for novel hydrolytic enzymes hydrolyzing polymeric compounds.
LanguageEnglish
JournalApplied and environmental microbiology
DOIs
StatusE-pub ahead of print - 9 Dec 2016

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Bryophyta
Esterases
esterases
moss
mosses and liverworts
microorganism
enzyme
microorganisms
substrate
pollution
Ecosystem
ecosystem
bog
Sphagnum magellanicum
Sphagnopsida
ester
microbial community
hydrolysis
recycling
Wetlands

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Discovery of Polyesterases from Moss-Associated Microorganisms. / Müller, Christina Andrea; Perz, Veronika; Provasnek, Christoph; Quartinello, Felice; Guebitz, Georg M.; Berg, Gabriele.

In: Applied and environmental microbiology, 09.12.2016.

Research output: Contribution to journalArticle

Müller, Christina Andrea ; Perz, Veronika ; Provasnek, Christoph ; Quartinello, Felice ; Guebitz, Georg M. ; Berg, Gabriele. / Discovery of Polyesterases from Moss-Associated Microorganisms. In: Applied and environmental microbiology. 2016
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