Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Class VIII self-sufficient CYP from Aspergillus fumigatus (CYP505X) and a variant with five mutations were heterologously expressed in Pichia pastoris. Ibuprofen – a widely used non-steroidal anti-inflammatory drug (NSAID) – was oxidized most efficiently in a whole cell mediated biotransformation. The two major metabolites were isolated by preparative HPLC and their structure elucidated by NMR. Specifically, ibuprofen was hydroxylated at the tertiary carbon atom and the benzylic position on the lipophilic side chain.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalBiocatalysis and Agricultural Biotechnology
Volume17
DOIs
Publication statusPublished - 2019

Fingerprint

ibuprofen
Hydroxylation
Pichia pastoris
Biocatalysts
Pichia
Aspergillus
Ibuprofen
hydroxylation
Metabolites
cytochrome P-450
Cytochrome P-450 Enzyme System
Nuclear magnetic resonance
Atoms
Carbon
Aspergillus fumigatus
nonsteroidal anti-inflammatory agents
Enzymes
Biotransformation
biotransformation
Anti-Inflammatory Agents

Keywords

  • API Metabolism
  • Cytochrome P450
  • Hydroxylation
  • Ibuprofen

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Food Science
  • Agronomy and Crop Science
  • Biotechnology

Fields of Expertise

  • Human- & Biotechnology

Cite this

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst. / Rinnofner, Claudia; Kerschbaumer, Bianca; Weber, Hansjörg; Glieder, Anton; Winkler, Margit.

In: Biocatalysis and Agricultural Biotechnology, Vol. 17, 2019, p. 525-528.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Winkler, Margit

PY - 2019

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