Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

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Abstract

Class VIII self-sufficient CYP from Aspergillus fumigatus (CYP505X) and a variant with five mutations were heterologously expressed in Pichia pastoris. Ibuprofen – a widely used non-steroidal anti-inflammatory drug (NSAID) – was oxidized most efficiently in a whole cell mediated biotransformation. The two major metabolites were isolated by preparative HPLC and their structure elucidated by NMR. Specifically, ibuprofen was hydroxylated at the tertiary carbon atom and the benzylic position on the lipophilic side chain.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalBiocatalysis and Agricultural Biotechnology
Volume17
DOIs
Publication statusPublished - 2019

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Keywords

  • API Metabolism
  • Cytochrome P450
  • Hydroxylation
  • Ibuprofen

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Food Science
  • Agronomy and Crop Science
  • Biotechnology

Fields of Expertise

  • Human- & Biotechnology

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