Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase

Thomas Kickenweiz, Anton Glieder, Jin Chuan Wu

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cellulose is a highly available and renewable carbon source in nature. However, it cannot be directly metabolized by most microbes including Komagataella phaffii (formerly Pichia pastoris), which is a frequently employed host for heterologous protein expression and production of high-value compounds. A K. phaffii strain was engineered that constitutively co-expresses an endoglucanase and a β-glucosidase both from Aspergillus niger and an exoglucanase from Trichoderma reesei under the control of bidirectional promoters. This engineered strain was able to grow on cellobiose and carboxymethyl cellulose (CMC) but not on Avicel. However, the detected release of cellobiose from Avicel by using the produced mixture of endoglucanase and exoglucanase as well as the released glucose from Avicel by using the produced mixture of all three cellulases at 50 °C indicated the production of exoglucanase under the liquid culture conditions. The successful expression of three cellulases in K. phaffii demonstrated the feasibility to enable K. phaffii to directly use cellulose as a carbon source for producing recombinant proteins or other high-value compounds.

Original languageEnglish
Pages (from-to)1297-1306
JournalApplied Microbiology and Biotechnology
Volume102
Issue number3
DOIs
Publication statusPublished - 2018

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Glucosidases
Pichia
Cellulose
Cellobiose
Cellulases
Cellulase
Carbon
Carboxymethylcellulose Sodium
Trichoderma
Aspergillus niger
Recombinant Proteins
Glucose
Proteins

Keywords

  • Journal Article

Cite this

Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase. / Kickenweiz, Thomas; Glieder, Anton; Wu, Jin Chuan.

In: Applied Microbiology and Biotechnology, Vol. 102, No. 3, 2018, p. 1297-1306.

Research output: Contribution to journalArticleResearchpeer-review

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