TY - JOUR
T1 - Confocal Luminescence Lifetime Imaging with Variable Scan Velocity and Its Application to Oxygen Sensing
AU - Petrášek, Zdeněk
AU - Bolivar Bolivar, Juan Manuel
AU - Nidetzky, Bernd
PY - 2016/11/1
Y1 - 2016/11/1
N2 - The dependence of the luminescence lifetime on the probe environment is the basis of a range of sensing techniques. The major advantage of using the lifetime as the sensitive parameter is its independence on the probe concentration. However, the instrumentation for lifetime measurements is complex, generally requiring time-resolved excitation and detection. Here, we present a simple method for the measurement of luminescence lifetimes on the microsecond scale based on variable excitation time determined by the scanning velocity. The technique is implemented in a confocal laser scanning microscope (CLSM), thus allowing not only simple lifetime measurement but also phosphorescence lifetime imaging. Since the method exploits the spatiotemporal dependence of sample excitation in a CLSM, there is no need for a pulsed or modulated light source or for additional time-resolved detection. The method can be realized in a standard CLSM without any modifications. The principle is demonstrated on oxygen sensing by collisional quenching of an oxygen-sensitive ruthenium(II) complex.
AB - The dependence of the luminescence lifetime on the probe environment is the basis of a range of sensing techniques. The major advantage of using the lifetime as the sensitive parameter is its independence on the probe concentration. However, the instrumentation for lifetime measurements is complex, generally requiring time-resolved excitation and detection. Here, we present a simple method for the measurement of luminescence lifetimes on the microsecond scale based on variable excitation time determined by the scanning velocity. The technique is implemented in a confocal laser scanning microscope (CLSM), thus allowing not only simple lifetime measurement but also phosphorescence lifetime imaging. Since the method exploits the spatiotemporal dependence of sample excitation in a CLSM, there is no need for a pulsed or modulated light source or for additional time-resolved detection. The method can be realized in a standard CLSM without any modifications. The principle is demonstrated on oxygen sensing by collisional quenching of an oxygen-sensitive ruthenium(II) complex.
UR - http://www.scopus.com/inward/record.url?scp=84993968510&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.6b03363
DO - 10.1021/acs.analchem.6b03363
M3 - Article
AN - SCOPUS:84993968510
SN - 0003-2700
VL - 88
SP - 10736
EP - 10743
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 21
ER -