Projects per year
Abstract
Biological membranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation, which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
Original language | English |
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Pages (from-to) | 1769-1776 |
Number of pages | 8 |
Journal | Biochimica et Biophysica Acta - Biomembranes |
Volume | 1838 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- Electron paramagnetic resonance
- Fluorescence correlation spectroscopy
- Lateral diffusion
- Molecular dynamics simulations
- Oxidized lipids
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology
Fields of Expertise
- Human- & Biotechnology
Treatment code (Nähere Zuordnung)
- Basic - Fundamental (Grundlagenforschung)
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Dive into the research topics of 'Comprehensive portrait of cholesterol containing oxidized membrane'. Together they form a unique fingerprint.Projects
- 1 Finished
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Protein targets of oxidized phospholipids relevant to apoptotic signalling of acid sphingomyelinase
Zenzmaier, E., Kayer, H., Lehsl, A., Vogl, F. & Hermetter, A.
1/10/09 → 30/09/14
Project: Research project