Comparative High-Resolution Transcriptome Sequencing of Lymphoma Cell Lines and de novo Lymphomas Reveals Cell-Line-Specific Pathway Dysregulation

Leila Taher, Julia Beck, Wen Liu, Catrin Roolf, Jan T Soller, Barbara C Rütgen, Sabine E Hammer, Murali Chodisetti, Sina Sender, Katharina A Sterenczak, Georg Fuellen, Christian Junghanss, Bertram Brenig, Ingo Nolte, Ekkehard Schütz, Hugo Murua Escobar

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In dogs as well as humans, lymphoma is one of the most common hematopoietic malignancies. Furthermore, due to its characteristics, canine lymphoma is recognized as a clinically relevant in vivo model to study the corresponding human disease. Immortalized cell lines are widely used as in vitro models to evaluate novel therapeutic agents and characterize their molecular mechanisms. However, it is known that long-term cultivation leads to clonal selection, genetic instability, and loss of the initial heterogenic character, limiting the usefulness of cell lines as preclinical models. Herein, we present a systematic characterization and comparison of the transcriptomic landscape of canine primary B- and T-cell lymphomas, five lymphoid cell lines (CLBL-1, CLBL-1M, GL-1, CL-1, and OSW) and four non-neoplastic control samples. We found that lymphomas and cell lines exhibit a common "differentiation and proliferation signature". However, our analysis also showed that, independently of the cell of origin, the transcriptional signatures of lymphomas are more similar to each other than they are to those of cell lines. In particular, we observed that not all common therapeutic targets are similarly expressed between lymphomas and lymphoid cell lines, and provide evidence that different lymphoid cell-lines should be used to model distinct aspects of lymphoma dysregulation.

Original languageEnglish
Pages (from-to)6279
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 19 Apr 2018

Keywords

  • Animals
  • Cell Differentiation/genetics
  • Cell Line, Tumor
  • Cell Proliferation/genetics
  • Disease Models, Animal
  • Dog Diseases/classification
  • Dogs
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lymphoma/classification
  • Receptors, Antigen, B-Cell/metabolism
  • Signal Transduction
  • Transcriptome/genetics
  • Whole Exome Sequencing/methods

Fingerprint Dive into the research topics of 'Comparative High-Resolution Transcriptome Sequencing of Lymphoma Cell Lines and de novo Lymphomas Reveals Cell-Line-Specific Pathway Dysregulation'. Together they form a unique fingerprint.

  • Cite this