Combination of Multidimensional Instrumental Analysis and the Ames Test for the Toxicological Evaluation of Mineral Oil Aromatic Hydrocarbons

Andrea Hochegger*, Reinhard Wagenhofer, Sanja Savic, Elisa Mayrhofer, Michael Washüttl, Erich Leitner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Mineral oil aromatic hydrocarbons (MOAHs) include mutagenic and carcinogenic substances and are considered a potential health risk. Current methods address the total MOAH content but cannot address the actual toxicological hazard of individual components. This work presents a combined methodology closing those gaps: high-performance liquid chromatography (HPLC) coupled to gas chromatography with flame ionization detection was used to determine the MOAH content. To characterize present substance classes, comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry was applied. Preparative HPLC separated MOAHs into subgroups, which were tested with a miniaturized Ames test evaluating DNA reactivity of isolated fractions. Combining these methods allowed a correlation between present subgroups and DNA reactivity. The developed approach was applied to a mineral oil and distinguished between not DNA-reactive mono- and diaromatics and DNA-reactive tri- and polyaromatics, providing a proof of concept. Hereinafter, it will be applied to diverse sample matrices including mineral oils, food, and food contact materials.
Original languageEnglish
Pages (from-to)16401–16409
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume70
Issue number51
DOIs
Publication statusPublished - 28 Dec 2022

Keywords

  • MOAH, HPLC-GC-FID, GC × GC-ToF, Ames assay, miniaturized Ames assay
  • GC × GC-ToF
  • HPLC-GC-FID
  • Ames assay
  • miniaturized Ames assay
  • MOAH

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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