Collapse of the native structure caused by a single amino acid exchange in human NAD(P)H:quinone oxidoreductase1

Wolf-Dieter Lienhart, Venugopal Gudipati, Michael K. Uhl, Alexandra Binter, Sergio A. Pulido, Robert Saf, Klaus Zangger, Karl Gruber, Peter Macheroux

Research output: Contribution to journalArticle

Abstract

Human NAD(P)H:quinone oxidoreductase 1 (NQO1) is essential for the antioxidant defense system, stabilization of tumor suppressors (e.g. p53, p33, and p73), and activation of quinone‐based chemotherapeutics. Overexpression of NQO1 in many solid tumors, coupled with its ability to convert quinone‐based chemotherapeutics into potent cytotoxic compounds, have made it a very attractive target for anticancer drugs. A naturally occurring single‐nucleotide polymorphism (C609T) leading to an amino acid exchange (P187S) has been implicated in the development of various cancers and poor survival rates following anthracyclin‐based adjuvant chemotherapy. Despite its importance for cancer prediction and therapy, the exact molecular basis for the loss of function in NQO1 P187S is currently unknown. Therefore, we solved the crystal structure of NQO1 P187S. Surprisingly, this structure is almost identical to NQO1. Employing a combination of NMR spectroscopy and limited proteolysis experiments, we demonstrated that the single amino acid exchange destabilized interactions between the core and C‐terminus, leading to depopulation of the native structure in solution. This collapse of the native structure diminished cofactor affinity and led to a less competent FAD‐binding pocket, thus severely compromising the catalytic capacity of the variant protein. Hence, our findings provide a rationale for the loss of function in NQO1 P187S with a frequently occurring single‐nucleotide polymorphism
Original languageEnglish
Pages (from-to)4691-4704
JournalThe FEBS Journal
Volume281
Issue number20
DOIs
Publication statusPublished - Sep 2014

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