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Abstract
Addressing the challenges associated with the development of in vitro biocatalytic carboxylate reductions for potential applications, important aspects of the co-factor regeneration systems and strategies for minimizing over-reduction were investigated. The ATP recycling can be performed with similarly high efficiency exploiting the polyphosphate source by combining Meiothermus ruber polyphosphate kinase and adenylate kinase or with Sinorhizobium meliloti polyphosphate kinase instead of the latter. Carboxylate reductions with the enzyme candidates used in this work allow operating at co-factor concentrations of adenosine 5'-triphosphate and β-nicotinamide adenine dinucleotide 2'-phosphate of 100 µM and, thereby, reducing the amounts of alcohols formed by side activities in the enzyme preparations. This study confirmed the expected benefits of carboxylic acid reductases in chemoselectively reducing the carboxylates to the corresponding aldehydes while leaving reductively-sensitive nitro, ester and cyano groups intact.
Original language | English |
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Pages (from-to) | 202-207 |
Number of pages | 6 |
Journal | Journal of Biotechnology |
Volume | 307 |
DOIs | |
Publication status | Published - 10 Jan 2020 |
Keywords
- Aldehyde
- Carboxylate reductase
- Cell-free ATP recycling
- Multi-enzyme cascade
- Polyphosphate
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Bioengineering
- Biotechnology
Fields of Expertise
- Human- & Biotechnology
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Dive into the research topics of 'Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems'. Together they form a unique fingerprint.Activities
- 1 Invited talk at conference or symposium
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Enzymatische Reduktion von (Fett)Säuren
Margit Winkler (Speaker)
7 Nov 2019Activity: Talk or presentation › Invited talk at conference or symposium › Science to science