Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris

Kristína Markošová, Andrea Camattari, Michal Rosenberg, Anton Glieder, Nicholas J Turner, Martin Rebroš

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To clone monoamine oxidase N, that catalyses the selective oxidative deamination or deracemisation of amines into imines, in Pichia pastoris and prove the importance of choosing the proper expression system for its recombinant production.

RESULTS: Monoamine oxidase, originating from Aspergillus niger and subjected to directed evolution (MAO-N D5), was cloned and expressed in Pichia pastoris CBS7435 MutS strain for the first time. Various transformants were screened at microscale level. The production of the clone expressing the most active enzyme was scaled-up to a 1.5 l fermenter and preparation of MAO-N D5 as a crude enzyme extract was optimised. The obstacles in the production of the enzyme in both expression systems, Escherichia coli and P. pastoris, are discussed and demonstrated.

CONCLUSIONS: There was an improvement in specific productivity, which was 83 times higher in P. pastoris, clearly proving the importance of choosing the right expression host system for the specific enzymes.

Original languageEnglish
Article numberhttps://doi.org/10.1007/s10529-017-2450-y
JournalBiotechnology Letters
Early online date10 Oct 2017
DOIs
Publication statusPublished - 2017

Keywords

  • Journal Article

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  • NAWI Graz

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    Markošová, K., Camattari, A., Rosenberg, M., Glieder, A., Turner, N. J., & Rebroš, M. (2017). Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris. Biotechnology Letters, [https://doi.org/10.1007/s10529-017-2450-y]. https://doi.org/10.1007/s10529-017-2450-y