Circulating cell-free DNA is predominantly composed of retrotransposable elements and non-telomeric satellite DNA

Stefan Grabuschnig, Jung Soh, Petra Heidinger, Thorsten Bachler, Elisabeth Hirschböck, Ingund Rosales Rodriguez, Daniel Schwendenwein, Christoph W Sensen*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Circulating cell-free DNAs (cfDNAs) are DNA fragments which can be isolated from mammalian blood serum or plasma. In order to gain deeper insight into their origin(s), we have characterized the composition of human and cattle cfDNA via large-scale analyses of high-throughput sequencing data. We observed significant differences between the composition of cfDNA in serum/plasma and the corresponding DNA sequence composition of the human genome. Retrotransposable elements and non-telomeric satellite DNA were particularly overrepresented in the cfDNA population, while telomeric satellite DNA was underrepresented. This was consistently observed for human plasma, bovine serum and for the supernatant of human cancer cell cultures. Our results suggest that reverse transcription of retrotransposable elements and secondary-structure formation during the replication of satellite DNA are contributing to the composition of the cfDNA molecules in the mammalian blood stream. We believe that our work is an important step towards the understanding of the biogenesis of cfDNAs and thus may also facilitate the future exploitation of their diagnostic potential.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalJournal of Biotechnology
Volume313
Early online date9 Mar 2020
DOIs
Publication statusPublished - 10 Apr 2020

Keywords

  • Active release
  • Cell-free DNA
  • Circulating nucleic acids
  • Exosomes
  • Retrotransposable elements
  • Satellite DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

Fields of Expertise

  • Human- & Biotechnology
  • Information, Communication & Computing

Treatment code (Nähere Zuordnung)

  • Application

Fingerprint Dive into the research topics of 'Circulating cell-free DNA is predominantly composed of retrotransposable elements and non-telomeric satellite DNA'. Together they form a unique fingerprint.

  • Cite this