Characterization of an anaerobic population digesting a model substrate for maize in the presence of trace metals

Herbert Pobeheim, Bernhard Munk, Henry Müller, Gabriele Berg, Georg Gübitz

Research output: Contribution to journalArticle

Abstract

The influence of a defined trace metal solution and additionally Ni2+ on anaerobic digestion of biomass was investigated. A novel synthetic model substrate was designed consisting of cellulose, starch and urea as carbon and nitrogen source in a ratio mimicking the basic composition of maize silage. Two independent batch fermentations were carried out over 21 d with the synthetic model substrate in the presence of the trace metal solution. Particularly an increase in nickel concentrations (17 and 34 μM) enhanced methane formation by up to 20%. This increased activity was also corroborated by fluorescence microscopy measurements based on cofactor F420. The eubacterial and methanogenic population was characterized with the single strand conformational polymorphism analysis and the amplified 16S rDNA restriction analysis of 16S rRNA genes amplified by different primer systems. Nearly the half of the …
Original languageEnglish
Pages (from-to)829-836
JournalChemosphere
DOIs
Publication statusPublished - 2010

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