TY - CHAP
T1 - Intensification of Aqueous Two-phase Extraction for Protein Purification
AU - Brandenbusch, Christoph
AU - Zeiner, Tim
AU - Merz, Juliane
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Within today's downstream processing of therapeutic proteins, there is an imminent need for alternatives to chromatographic separations, such as aqueous two-phase extraction. Challenges in the development and performance of aqueous two-phase extraction include a number of experiments to define appropriate process windows as well as enable multistage extraction. To reduce experimental effort, a hybrid thermodynamic model is presented in this contribution which allows the extraction system to be optimized with a smaller number of experiments. Moreover, multistage applications of aqueous two-phase extraction are presented to purify enzymes. To purify the investigated enzymes, two different apparatuses were used: namely, a mixer-settler unit and a centrifugal partition chromatograph.
AB - Within today's downstream processing of therapeutic proteins, there is an imminent need for alternatives to chromatographic separations, such as aqueous two-phase extraction. Challenges in the development and performance of aqueous two-phase extraction include a number of experiments to define appropriate process windows as well as enable multistage extraction. To reduce experimental effort, a hybrid thermodynamic model is presented in this contribution which allows the extraction system to be optimized with a smaller number of experiments. Moreover, multistage applications of aqueous two-phase extraction are presented to purify enzymes. To purify the investigated enzymes, two different apparatuses were used: namely, a mixer-settler unit and a centrifugal partition chromatograph.
UR - http://www.scopus.com/inward/record.url?scp=85051546705&partnerID=8YFLogxK
U2 - 10.1039/9781788010320-00344
DO - 10.1039/9781788010320-00344
M3 - Chapter
AN - SCOPUS:85051546705
T3 - RSC Green Chemistry
SP - 344
EP - 364
BT - Catalyst-free Organic Synthesis
PB - Royal Society of Chemistry
ER -