Biocatalytic synthesis of optically active tertiary alcohols

R Kourist, U T Bornscheuer

Research output: Contribution to journalArticle

Abstract

The enzymatic preparation of optically pure tertiary alcohols under sustainable conditions has received much attention. The conventional chemical synthesis of these valuable building blocks is still hampered by the use of harmful reagents such as heavy metal catalysts. Successful examples in biocatalysis used esterases, lipases, epoxide hydrolases, halohydrin dehalogenases, thiamine diphosphate-dependent enzymes, terpene cyclases, -acetylases, and -dehydratases. This mini-review provides an overview on recent developments in the discovery of new enzymes, their functional improvement by protein engineering, the design of chemoenzymatic routes leading to tertiary alcohols, and the discovery of entirely new biotransformations.

LanguageEnglish
Pages505-17
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume91
Issue number3
DOIs
StatusPublished - 2011

Fingerprint

Alcohols
Biocatalysis
Acetylesterase
Hydro-Lyases
Thiamine Pyrophosphate
Epoxide Hydrolases
Protein Engineering
Terpenes
Esterases
Enzymes
Biotransformation
Heavy Metals
Lipase
halohydrin dehalogenase

Keywords

  • Alcohols
  • Bacteria
  • Biocatalysis
  • Hydrolases
  • Protein Engineering
  • Terpenes
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review

Fields of Expertise

  • Human- & Biotechnology

Cite this

Biocatalytic synthesis of optically active tertiary alcohols. / Kourist, R; Bornscheuer, U T.

In: Applied Microbiology and Biotechnology, Vol. 91, No. 3, 2011, p. 505-17.

Research output: Contribution to journalArticle

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