Assessment of pharmacokinetics for microvessel proliferation by DCE-MRI for early detection of physeal bone bridge formation in an animal model

Bernhard Neumayer, Eva Amerstorfer, Clemens Diwoky, Richard A Lindtner, Elisabeth Wadl, Eva Scheurer, Annelie-Martina Weinberg, Rudolf Stollberger*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

OBJECTIVES: Bone bridge formation occurs after physeal lesions and can lead to growth arrest if not reversed. Previous investigations on the underlying mechanisms of this formation used histological methods. Therefore, this study aimed to apply a minimally invasive method using dynamic contrast-enhanced MRI (DCE-MRI).

MATERIALS AND METHODS: Changes in functional parameters related to the microvessel system were assessed in a longitudinal study of a cohort of an animal model applying a reference region model. The development of morphology of the injured physis was investigated with 3D high-resolution MRI. To acquire complementary information for MRI-related findings qRT-PCR and immunohistochemical data were acquired for a second cohort of the animal model.

RESULTS: The evaluation of the pharmacokinetic parameters showed a first rise of the transfer coefficient 7 days post-lesion and a maximum 42 days after operation. The analysis of the complementary data showed a connection of the first rise to microvessel proliferation while the maximum value was linked to bone remodeling.

CONCLUSION: The pharmacokinetic analysis of DCE-MRI provides information on a proliferation of microvessels during the healing process as a sign for bone bridge formation. Thereby, DCE-MRI could identify details, which up to now required analyses of highly invasive methods.

Original languageEnglish
Pages (from-to)417-427
Number of pages11
JournalMagnetic Resonance Materials in Physics, Biology and Medicine
Volume30
Issue number5
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Journal Article

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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