Analysis of galactoglucomannans from spruce wood by capillary electrophoresis

Aleš Doliška, Simona Strnad, Volker Ribitsch, Karin Stana Kleinschek, Stefan Willför, Bodo Saake

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The aim of this study was to setup a method for detection and quantification of monosaccharide components in technical galactoglucomannas (T-GGM) from spruce wood using capillary zone electrophoresis (CZE). CZE technique was optimised regarding borate buffer concentrations, EOF modifier application, and system pH. Aqueous solution of T-GGM was chemically hydrolysed by sulphuric acid, in an autoclave. In this way obtained monosaccharides were derivatized with 4-amino benzoic acid ethyl ester via reductive amination using sodium cyanoborohydride. The results of the optimisation procedure showed that the borate buffers at lowest concentrations (100 and 200 mM) with acetonitrile addition as EOF modifier gave the optimal measurement results, as it showed sufficient separation at relatively short migration times. The amounts of single monosaccharide components in the T-GGM samples obtained by the optimised CZE procedure were practically the same in comparison to the results of the well established HPLC-anion exchange chromatography. On the basis of this research, it was concluded that the capillary zone electrophoresis is an efficient analytical procedure for the characterisation of galactoglucomannans derived from softwoods.

Original languageEnglish
Pages (from-to)1089-1097
Number of pages9
JournalCellulose
Volume16
Issue number6
DOIs
Publication statusPublished - 1 Dec 2009
Externally publishedYes

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Keywords

  • Capillary electrophoresis
  • Carbohydrates
  • Galactoglucomannan
  • Glucomannan
  • Polysaccharide

ASJC Scopus subject areas

  • Polymers and Plastics

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