Abstract
Over the last two decades, activity-based protein profiling (ABPP) has been established as a tremendously useful proteomic tool for measuring the activity of proteins in their cellular context, annotating the function of uncharacterized proteins, and investigating the target profile of small-molecule inhibitors. Unlike hydrolases and other enzyme classes, which exhibit a characteristic nucleophilic residue, oxidoreductases have received much less attention in ABPP. In this minireview, the state of the art of ABPP of oxidoreductases is described and the scope and limitations of the existing approaches are discussed. It is noted that several ABPP probes have been described for various oxidases, but none so far for a reductase, which gives rise to opportunities for future research.
Original language | English |
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Pages (from-to) | 630–638 |
Number of pages | 9 |
Journal | ChemBioChem |
Volume | 22 |
Issue number | 4 |
Early online date | 2 Sept 2020 |
DOIs | |
Publication status | Published - 15 Feb 2021 |
Keywords
- chemical proteomics
- covalent inhibitors
- drug discovery
- oxidoreductases
- proteomics
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Biochemistry
- Organic Chemistry