Activity‐Based Protein Profiling (ABPP) of Oxidoreductases

Rita Fürst, Rolf Breinbauer*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

Abstract

Over the last two decades, activity-based protein profiling (ABPP) has been established as a tremendously useful proteomic tool for measuring the activity of proteins in their cellular context, annotating the function of uncharacterized proteins, and investigating the target profile of small-molecule inhibitors. Unlike hydrolases and other enzyme classes, which exhibit a characteristic nucleophilic residue, oxidoreductases have received much less attention in ABPP. In this minireview, the state of the art of ABPP of oxidoreductases is described and the scope and limitations of the existing approaches are discussed. It is noted that several ABPP probes have been described for various oxidases, but none so far for a reductase, which gives rise to opportunities for future research.

Original languageEnglish
Pages (from-to)630–638
Number of pages9
JournalChemBioChem
Volume22
Issue number4
Early online date2 Sep 2020
DOIs
Publication statusPublished - 15 Feb 2021

Keywords

  • chemical proteomics
  • covalent inhibitors
  • drug discovery
  • oxidoreductases
  • proteomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry

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