Activated retinal glia promote neurite outgrowth of retinal ganglion cells via apolipoprotein E

Barbara Lorber; Martin Berry; Michael R Douglas; Toru Nakazawa; Ann Logan

doi:10.1002/jnr.22095

Activated retinal glia promote neurite outgrowth of retinal ganglion cells via apolipoprotein E

Barbara Lorber, Martin Berry, Michael R Douglas, Toru Nakazawa, Ann Logan

Research output: Contribution to journal › Article › peer-review

Abstract

In the present study, we investigated the role and mechanism through which activated retinal glia stimulate retinal ganglion cell (RGC) neurite outgrowth. We have found that the level of retinal glial activation correlates directly with enhanced RGC neurite outgrowth after a preconditioning intravitreal Zymosan injection. Reduction in the number of activated glia in primary rat retinal cultures led to significantly reduced RGC neurite outgrowth. Glial-related neurite outgrowth appears to be, at least in part, mediated via apolipoprotein E (ApoE), which is expressed by activated retinal astrocytes and Müller glia. ApoE-deficient mice showed significantly reduced RGC neurite outgrowth potential after intravitreal Zymosan injection compared with age-matched wild-type animals. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection.

Original language	English
Pages (from-to)	2645-52
Number of pages	8
Journal	Journal of Neuroscience Research
Volume	87
Issue number	12
DOIs	https://doi.org/10.1002/jnr.22095
Publication status	Published - Sept 2009
Externally published	Yes

Keywords

Apolipoproteins E
Cells, Cultured
Glial Fibrillary Acidic Protein
Transgenics
Nerve Regeneration
Neurites
Neuroglia
Optic Nerve
Retinal Ganglion Cells
Zymosan

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title = "Activated retinal glia promote neurite outgrowth of retinal ganglion cells via apolipoprotein E",

abstract = "In the present study, we investigated the role and mechanism through which activated retinal glia stimulate retinal ganglion cell (RGC) neurite outgrowth. We have found that the level of retinal glial activation correlates directly with enhanced RGC neurite outgrowth after a preconditioning intravitreal Zymosan injection. Reduction in the number of activated glia in primary rat retinal cultures led to significantly reduced RGC neurite outgrowth. Glial-related neurite outgrowth appears to be, at least in part, mediated via apolipoprotein E (ApoE), which is expressed by activated retinal astrocytes and M{\"u}ller glia. ApoE-deficient mice showed significantly reduced RGC neurite outgrowth potential after intravitreal Zymosan injection compared with age-matched wild-type animals. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection.",

keywords = "Apolipoproteins E, Cells, Cultured, Glial Fibrillary Acidic Protein, Transgenics, Nerve Regeneration, Neurites, Neuroglia, Optic Nerve, Retinal Ganglion Cells, Zymosan",

author = "Barbara Lorber and Martin Berry and Douglas, {Michael R} and Toru Nakazawa and Ann Logan",

year = "2009",

month = sep,

doi = "10.1002/jnr.22095",

language = "English",

volume = "87",

pages = "2645--52",

journal = "Journal of Neuroscience Research",

issn = "0360-4012",

publisher = "Wiley-Blackwell",

number = "12",

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TY - JOUR

T1 - Activated retinal glia promote neurite outgrowth of retinal ganglion cells via apolipoprotein E

AU - Lorber, Barbara

AU - Berry, Martin

AU - Douglas, Michael R

AU - Nakazawa, Toru

AU - Logan, Ann

PY - 2009/9

Y1 - 2009/9

N2 - In the present study, we investigated the role and mechanism through which activated retinal glia stimulate retinal ganglion cell (RGC) neurite outgrowth. We have found that the level of retinal glial activation correlates directly with enhanced RGC neurite outgrowth after a preconditioning intravitreal Zymosan injection. Reduction in the number of activated glia in primary rat retinal cultures led to significantly reduced RGC neurite outgrowth. Glial-related neurite outgrowth appears to be, at least in part, mediated via apolipoprotein E (ApoE), which is expressed by activated retinal astrocytes and Müller glia. ApoE-deficient mice showed significantly reduced RGC neurite outgrowth potential after intravitreal Zymosan injection compared with age-matched wild-type animals. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection.

AB - In the present study, we investigated the role and mechanism through which activated retinal glia stimulate retinal ganglion cell (RGC) neurite outgrowth. We have found that the level of retinal glial activation correlates directly with enhanced RGC neurite outgrowth after a preconditioning intravitreal Zymosan injection. Reduction in the number of activated glia in primary rat retinal cultures led to significantly reduced RGC neurite outgrowth. Glial-related neurite outgrowth appears to be, at least in part, mediated via apolipoprotein E (ApoE), which is expressed by activated retinal astrocytes and Müller glia. ApoE-deficient mice showed significantly reduced RGC neurite outgrowth potential after intravitreal Zymosan injection compared with age-matched wild-type animals. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection.

KW - Apolipoproteins E

KW - Cells, Cultured

KW - Glial Fibrillary Acidic Protein

KW - Transgenics

KW - Nerve Regeneration

KW - Neurites

KW - Neuroglia

KW - Optic Nerve

KW - Retinal Ganglion Cells

KW - Zymosan

U2 - 10.1002/jnr.22095

DO - 10.1002/jnr.22095

M3 - Article

C2 - 19382209

SN - 0360-4012

VL - 87

SP - 2645

EP - 2652

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

IS - 12

ER -

Activated retinal glia promote neurite outgrowth of retinal ganglion cells via apolipoprotein E

Abstract

Keywords

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