Abstract
An efficient 2′-O- to 3′-C-β-D-glucosidic bond rearrangement on the dihydrochalcone phloretin to convert phlorizin into nothofagin was achieved by combining complementary O-glycosyltransferase (OGT) and C-glycosyltransferase (CGT) activities in a one-pot transformation containing catalytic amounts of uridine 5′-diphosphate (UDP). Two separate enzymes or a single engineered dual-specific O/CGT were applied. Overall (quantitative) conversion occurred in two steps via intermediary UDP–glucose and phloretin.
Original language | English |
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Pages (from-to) | 5465-5468 |
Journal | Chemical Communications |
Volume | 50 |
Issue number | 41 |
DOIs | |
Publication status | Published - 2014 |
Fields of Expertise
- Human- & Biotechnology