A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Geoffrey Gourinchas; Eduardo Busto; Manuela Killinger; Nina Richter; Birgit Wiltschi; Wolfgang Kroutil

doi:10.1039/c4cc08286a

A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Geoffrey Gourinchas, Eduardo Busto, Manuela Killinger, Nina Richter, Birgit Wiltschi^*, Wolfgang Kroutil^*

^*Corresponding author for this work

Graz University of Technology (90000)

Research output: Contribution to journal › Article › peer-review

Abstract

Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.

Original language	English
Pages (from-to)	2828-2831
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	14
DOIs	https://doi.org/10.1039/c4cc08286a
Publication status	Published - 9 Jan 2015

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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AU - Richter, Nina

AU - Wiltschi, Birgit

AU - Kroutil, Wolfgang

PY - 2015/1/9

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AB - Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.

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A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Abstract

ASJC Scopus subject areas

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