A One-Pot Cascade Reaction Combining an Encapsulated Decarboxylase with a Metathesis Catalyst for the Synthesis of Bio-Based Antioxidants

Álvaro Gómez Baraibar, Dennis Reichert, Carolin Mügge, Svenja Seger, Harald Gröger, Robert Kourist

Research output: Contribution to journalArticleResearchpeer-review


The combination of enzymes with traditional chemical catalysts unifies the high selectivity of the former with the versatility of the latter. A major challenge of this approach is the difference in the optimal reaction conditions for each catalyst type. In this work, we combined a cofactor-free decarboxylase with a ruthenium metathesis catalyst to produce high-value antioxidants from bio-based precursors. As suitable ruthenium catalysts did not show satisfactory activity under aqueous conditions, the reaction required the use of an organic solvent, which in turn significantly reduced enzyme activity. Upon encapsulation of the decarboxylase in a cryogel, the decarboxylation could be conducted in an organic solvent, and the recovery of the enzyme after the reaction was facilitated. After an intermediate drying step, the subsequent metathesis in pure organic solvent proved to be straightforward. The synthetic utility of the cascade was demonstrated by the synthesis of the antioxidant 4,4′-dihydroxystilbene in an overall yield of 90 %.

Original languageEnglish
Pages (from-to)14823-14827
Number of pages5
JournalAngewandte Chemie / International Edition
Issue number47
Publication statusPublished - 14 Nov 2016
Externally publishedYes



  • bio-based antioxidants
  • biocatalysis
  • compartmentalization
  • decarboxylation
  • metathesis

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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