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• 2020

  A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 May 2020, In : ACS Central Science. 6, 5, p. 739-746 8 p.

  Research output: Contribution to journal › Article

  Open Access

  • Cellulose
  • Crystalline materials
  • Nanocrystals
  • Cellulases
  • Degradation

• Enzyme Immobilization in Wall-Coated Flow Microreactors

  Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

  Research output: Contribution to journal › Article

  • Immobilization
  • Enzymes
  • sucrose phosphorylase
  • Silicon Dioxide
  • Glass

• Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

  Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

  Research output: Contribution to journal › Article

  • Immobilized Enzymes
  • Proteins
  • Enzymes
  • Penicillin Amidase
  • Proton-Motive Force

• Magnetically responsive horseradish peroxidase@ZIF-8 for biocatalysis

  Ricco, R., Wied, P., Nidetzky, B., Amenitsch, H. & Falcaro, P., 28 May 2020, In : Chemical Communications. 56, 43, p. 5775-5778 4 p.

  Research output: Contribution to journal › Article

  Open Access

  • Biocatalysis
  • Horseradish Peroxidase
  • Metal Nanoparticles
  • Enzymes
  • Ions

• Model-based analysis of biocatalytic processes and performance of microbioreactors with integrated optical sensors

  Semenova, D., Fernandes, A. C., Bolivar, J. M., Rosinha Grundtvig, I. P., Vadot, B., Galvanin, S., Mayr, T., Nidetzky, B., Zubov, A. & Gernaey, K. V., 2020, In : New Biotechnology. 56, p. 27-37 11 p.

  Research output: Contribution to journal › Article

  • Microfluidics
  • Optical sensors
  • Biocatalysis
  • Glucose Oxidase
  • Screening

• On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

  Bolivar, J. M. & Nidetzky, B., 1 Feb 2020, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1868, 2, 140333.

  Research output: Contribution to journal › Review article

  • Immobilized Enzymes
  • Molecules
  • Enzymes
  • Immobilization
  • Catalytic Domain

• Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose.

  Schwaiger, K. N., Voit, A., Dobiašová, H., Luley, C., Wiltschi, B. & Nidetzky, B., Nov 2020, In : Biotechnology Journal. 15, 11, 2000063.

  Research output: Contribution to journal › Article

  Open Access

  • Cellobiose
  • Plasmids
  • Enzymes
  • cellobiose phosphorylase
  • sucrose phosphorylase

• Separation behavior and microstructure of emulsified, two-phasic E. coli bioreaction mixtures

  Lauß, B., Rapp, C., Leis, D., Nidetzky, B. & Kratzer, R., 2020, In : Colloid and Interface Science Communications. 35, 100248.

  Research output: Contribution to journal › Article
• 2019

  Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

  Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • cysteine
  • Gold
  • Cysteine
  • Monolayers
  • Desorption

• A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

  Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, In : ACS Catalysis. 9, 4, p. 2962-2968 7 p.

  Research output: Contribution to journal › Article

  Open Access

  • Guanosine Diphosphate Mannose
  • Hydro-Lyases
  • Dehydration
  • Sugars
  • Deoxy Sugars

• Comparative time-lapse AFM study of crystalline cellulose deconstruction by free cellulases and the cellulosome

  Seelich, K., Eibinger, M. & Nidetzky, B., Nov 2019.

  Research output: Contribution to conference › Poster

• Correction: Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, 1 p.

  Research output: Contribution to journal › Comment/debate

  Open Access

  • Hexosaminidases
  • synthesis
  • lacto-N-neotetraose
  • Bifidobacterium bifidum

• Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

  Savino, S., Borg, A. J. E., Dennig, A., Pfeiffer, M., De Giorgi, F., Weber, H., Dubey, K. D., Rovira, C., Mattevi, A. & Nidetzky, B., 1 Dec 2019, In : Nature Catalysis. 2, 12, p. 1115-1123 9 p.

  Research output: Contribution to journal › Article

  • Biosynthesis
  • Sugars
  • Xylose
  • Uridine Diphosphate
  • Decarboxylation

• Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

  Lemmerer, M., Mairhofer, J., Lepak, A., Longus, K., Hahn, R. & Nidetzky, B., 1 Jun 2019, In : Biotechnology and Bioengineering. 116, 6, p. 1259-1268 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • Recombinant proteins
  • Glycosyltransferases
  • Cell growth
  • Recombinant Proteins
  • Escherichia coli

• Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto- N-triose II Production with Engineered exo-Hexosaminidase

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 7 Jun 2019, In : ACS Catalysis. 9, 6, p. 5503-5514 12 p.

  Research output: Contribution to journal › Article

  • Hexosaminidases
  • Glucosamine
  • Glycosylation
  • Lactose
  • Trisaccharides

• Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

  Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

  Research output: Contribution to journal › Article

  Open Access

  • Nitriles
  • Proton transfer
  • Catalysis
  • Escherichia coli
  • Protons

• Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, p. 5661-5665 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • Hexosaminidases
  • Human Milk
  • Lactose
  • Oligosaccharides
  • Glycosylation

• Preparative Asymmetric Synthesis of Canonical and Non-canonical a-amino Acids through Formal Enantioselective Biocatalytic Amination of Carboxylic Acids

  Dennig, A., Blaschke, F., Gandomkar, S., Tassano, E. & Nidetzky, B., 1 Jan 2019, In : Advanced Synthesis and Catalysis. 361, 6, p. 1348-1358 11 p.

  Research output: Contribution to journal › Article

  Open Access

  • Amination
  • Carboxylic Acids
  • Carboxylic acids
  • Amino acids
  • Fatty acids

• Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

  Zhong, C., Luley-Goedl, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology and Bioengineering. 116, 9, p. 2146-2155 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • cellodextrin phosphorylase
  • cellobiose phosphorylase
  • Polymerization
  • Solubility
  • Glucose

• Sialic acid aldolase and synthase compared in optimized multi-enzyme cascade production of 3'-sialyllactose

  Schelch, S., Gross Belduma, S. M., Eibinger, M., Kuballa, J., Petschacher, B. & Nidetzky, B., 18 Nov 2019.

  Research output: Contribution to conference › Poster

• Sustainability assessment integrated in bioprocess design in H2020 Project CARBAFIN: When thinking about process optimization - are we also thinking about environmental aspects?

  Kollmann, R., Petschacher, B., Koenig, S., Kruschitz, A., Nidetzky, B. & Passer, A., 19 Nov 2019.

  Research output: Contribution to conference › Poster

• The influence of feedstock characteristics on enzyme production in Trichoderma reesei: A review on productivity, gene regulation and secretion profiles

  Novy, V., Nielsen, F., Seiboth, B. & Nidetzky, B., 1 Jan 2019, In : Biotechnology for Biofuels. 12, 1, 238.

  Research output: Contribution to journal › Review article

  Open Access

  • Trichoderma
  • Gene expression
  • secretion
  • Feedstocks
  • Enzymes

• The microenvironment in immobilized enzymes: Methods of characterization and its role in determining enzyme performance

  Bolivar, J. M. & Nidetzky, B., 24 Sep 2019, In : Molecules . 24, 19, 3460.

  Research output: Contribution to journal › Article

  Open Access

  • Immobilized Enzymes
  • enzymes
  • Enzymes
  • Liquids
  • Ionic strength

• Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

  Zhong, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology Journal. 1900349.

  Research output: Contribution to journal › Article

  Open Access

  • Phosphorylases
  • Sucrose
  • Enzymes
  • Polymerization
  • Phosphates
• 2017

  An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

  Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

  Research output: Contribution to journal › Article

• A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

  Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

  Research output: Contribution to journal › Article

  • Enzyme immobilization
  • Microfluidics
  • Silicon Dioxide
  • Enzymes
  • Silica

• Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

  Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

  Research output: Contribution to journal › Article

  Open Access

  • Sugar (sucrose)
  • Nucleotides
  • Nucleosides
  • Glucose
  • Diphosphates

• Debottlenecking enzymatic production of fucosylated HMOs with prokaryotic a1,2-fucosyltransferases

  Petschacher, B., Gierlinger, K., Brunner, L., Kuballa, J. & Nidetzky, B., 23 Apr 2017.

  Research output: Contribution to conference › Poster

• Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

  Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

  Research output: Contribution to journal › Article

  • Uridine Diphosphate Glucose
  • Glycosyltransferases
  • Uridine Diphosphate
  • Recycling
  • Biological Products

• Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

  Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, In : Journal of Biotechnology. 257, p. 110-117

  Research output: Contribution to journal › Article

• Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

  Research output: Contribution to journal › Article

  • Xylose
  • Uridine Diphosphate
  • Isotopes
  • Enzymes
  • Sugars

• Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

  Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, In : ChemCatChem. 9, 1, p. 161-166 6 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • sucrose
  • Sugar (sucrose)
  • microchannels
  • Microchannels

• Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

  Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, In : Journal of Biotechnology. 257, p. 131-138 8 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • Sugar (sucrose)
  • Glycerol
  • Catalyst supports
  • Enzymes

• Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

  Research output: Contribution to journal › Article

• Saccharomyces cerevisiae strain comparison in glucose-xylose fermentations on defined substrates and in high-gravity SSCF: Convergence in strain performance despite differences in genetic and evolutionary engineering history

  Novy, V., Wang, R., Westman, J. O., Franzén, C. J. & Nidetzky, B., 4 Sep 2017, In : Biotechnology for Biofuels. 10, 1, 205.

  Research output: Contribution to journal › Review article

  • Hypergravity
  • Xylose
  • Genetic Engineering
  • Yeast
  • Fermentation

• Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

  Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, In : Protein engineering, design and selection. 30, 3, p. 141-148

  Research output: Contribution to journal › Article

  • Uridine Diphosphate
  • Sugar (sucrose)
  • Nucleotides
  • Administrative data processing
  • Diphosphates

• Single-molecule study of oxidative enzymaticdeconstruction of cellulose

  Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, In : Nature Communications . 8, 7 p., 894.

  Research output: Contribution to journal › Article

  Open Access

  • cellulose
  • Cellulose
  • polysaccharides
  • Polysaccharides
  • Mixed Function Oxygenases

• Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

  Viefhues, M., Sun, S., Valikhani, D., Nidetzky, B., Vrouwe, E. X., Mayr, T. & Bolivar, J. M., 10 May 2017, In : Journal of Micromechanics and Microengineering. 27, 6, 065012.

  Research output: Contribution to journal › Article

  • Oxygen sensors
  • Immobilized Enzymes
  • Glucose Oxidase
  • Glucose oxidase
  • Optical sensors

• Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

  Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

  Research output: Contribution to journal › Article

  • Xylose
  • L-Lactate Dehydrogenase
  • Yeast
  • Fermentation
  • Glucose

• Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

  Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, In : ChemBioChem. 18, 14, p. 1387-1390 4 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • Ascorbic Acid
  • Sucrose
  • Cosmetics
  • Protonation
• 2015

  Action of an AA9 - type lytic polysaccharide monooxygenase on the cellulose surface and its effect on cellulase hydrolytic efficiency

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 2 Aug 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

  Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, In : Analytical biochemistry. 483, p. 47-53

  Research output: Contribution to journal › Article

• Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

  Ganner, T., Sattelkow, J., Rosker, S., Eibinger, M., Chernev, B. S., Fitzek, H. M., Kraxner, J., Mayrhofer, C., Aschl, T., Nidetzky, B. & Plank, H., 2015, ASEM Workshop Advanced Electron Microscopy. ., p. 30-30

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

  Ganner, T., Eibinger, M., Rosker, S., Sattelkow, J., Aschl, T., Rattenberger, J., Fitzek, H. M., Nidetzky, B. & Plank, H., 7 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• C-Glycosyltransferases: From mechanistic insight to synthetic application in flavonoid glycosylation

  Gutmann, A., Bungaruang, L. & Nidetzky, B., 5 Aug 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

  Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, In : Analytical biochemistry. 490, p. 46-51

  Research output: Contribution to journal › Article

• Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 10 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 4 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, In : Chemical Communications. p. 3083-3086

  Research output: Contribution to journal › Article

• Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, In : ChemBioChem. 16, 13, p. 1870-1874

  Research output: Contribution to journal › Article

  • Antioxidants
  • Water
  • Solubility
  • Glycosylation
  • Glucosyltransferases