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• 2019

  Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

  Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • cysteine
  • Gold
  • Cysteine
  • Monolayers
  • Desorption

• A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

  Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, In : ACS Catalysis. 9, 4, p. 2962-2968 7 p.

  Research output: Contribution to journal › Article

  Open Access

  • Guanosine Diphosphate Mannose
  • Hydro-Lyases
  • Dehydration
  • Sugars
  • Deoxy Sugars

• Comparative time-lapse AFM study of crystalline cellulose deconstruction by free cellulases and the cellulosome

  Seelich, K., Eibinger, M. & Nidetzky, B., Nov 2019.

  Research output: Contribution to conference › Poster

• Correction: Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, 1 p.

  Research output: Contribution to journal › Comment/debate

  Open Access

  • Hexosaminidases
  • synthesis
  • lacto-N-neotetraose
  • Bifidobacterium bifidum

• Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

  Savino, S., Borg, A. J. E., Dennig, A., Pfeiffer, M., De Giorgi, F., Weber, H., Dubey, K. D., Rovira, C., Mattevi, A. & Nidetzky, B., 1 Dec 2019, In : Nature Catalysis. 2, 12, p. 1115-1123 9 p.

  Research output: Contribution to journal › Article

  • Biosynthesis
  • Sugars
  • Xylose
  • Uridine Diphosphate
  • Decarboxylation

• Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

  Lemmerer, M., Mairhofer, J., Lepak, A., Longus, K., Hahn, R. & Nidetzky, B., 1 Jun 2019, In : Biotechnology and Bioengineering. 116, 6, p. 1259-1268 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • Recombinant proteins
  • Glycosyltransferases
  • Cell growth
  • Recombinant Proteins
  • Escherichia coli

• Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto- N-triose II Production with Engineered exo-Hexosaminidase

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 7 Jun 2019, In : ACS Catalysis. 9, 6, p. 5503-5514 12 p.

  Research output: Contribution to journal › Article

  • Hexosaminidases
  • Glucosamine
  • Glycosylation
  • Lactose
  • Trisaccharides

• Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

  Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

  Research output: Contribution to journal › Article

  Open Access

  • Nitriles
  • Proton transfer
  • Catalysis
  • Escherichia coli
  • Protons

• Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, p. 5661-5665 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • Hexosaminidases
  • Human Milk
  • Lactose
  • Oligosaccharides
  • Glycosylation

• Preparative Asymmetric Synthesis of Canonical and Non-canonical a-amino Acids through Formal Enantioselective Biocatalytic Amination of Carboxylic Acids

  Dennig, A., Blaschke, F., Gandomkar, S., Tassano, E. & Nidetzky, B., 1 Jan 2019, In : Advanced Synthesis and Catalysis. 361, 6, p. 1348-1358 11 p.

  Research output: Contribution to journal › Article

  Open Access

  • Amination
  • Carboxylic Acids
  • Carboxylic acids
  • Amino acids
  • Fatty acids

• Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

  Zhong, C., Luley-Goedl, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology and Bioengineering. 116, 9, p. 2146-2155 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • cellodextrin phosphorylase
  • cellobiose phosphorylase
  • Polymerization
  • Solubility
  • Glucose

• Sialic acid aldolase and synthase compared in optimized multi-enzyme cascade production of 3'-sialyllactose

  Schelch, S., Gross Belduma, S. M., Eibinger, M., Kuballa, J., Petschacher, B. & Nidetzky, B., 18 Nov 2019.

  Research output: Contribution to conference › Poster

• Sustainability assessment integrated in bioprocess design in H2020 Project CARBAFIN: When thinking about process optimization - are we also thinking about environmental aspects?

  Kollmann, R., Petschacher, B., Koenig, S., Kruschitz, A., Nidetzky, B. & Passer, A., 19 Nov 2019.

  Research output: Contribution to conference › Poster

• The influence of feedstock characteristics on enzyme production in Trichoderma reesei: A review on productivity, gene regulation and secretion profiles

  Novy, V., Nielsen, F., Seiboth, B. & Nidetzky, B., 1 Jan 2019, In : Biotechnology for Biofuels. 12, 1, 238.

  Research output: Contribution to journal › Review article

  Open Access

  • Trichoderma
  • Gene expression
  • secretion
  • Feedstocks
  • Enzymes

• The microenvironment in immobilized enzymes: Methods of characterization and its role in determining enzyme performance

  Bolivar, J. M. & Nidetzky, B., 24 Sep 2019, In : Molecules . 24, 19, 3460.

  Research output: Contribution to journal › Article

  Open Access

  • Immobilized Enzymes
  • enzymes
  • Enzymes
  • Liquids
  • Ionic strength

• Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

  Zhong, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology Journal. 1900349.

  Research output: Contribution to journal › Article

  Open Access

  • Phosphorylases
  • Sucrose
  • Enzymes
  • Polymerization
  • Phosphates
• 2017

  An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

  Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

  Research output: Contribution to journal › Article

• A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

  Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

  Research output: Contribution to journal › Article

  • Enzyme immobilization
  • Microfluidics
  • Silicon Dioxide
  • Enzymes
  • Silica

• Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

  Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

  Research output: Contribution to journal › Article

  Open Access

  • Sugar (sucrose)
  • Nucleotides
  • Nucleosides
  • Glucose
  • Diphosphates

• Debottlenecking enzymatic production of fucosylated HMOs with prokaryotic a1,2-fucosyltransferases

  Petschacher, B., Gierlinger, K., Brunner, L., Kuballa, J. & Nidetzky, B., 23 Apr 2017.

  Research output: Contribution to conference › Poster

• Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

  Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

  Research output: Contribution to journal › Article

  • Uridine Diphosphate Glucose
  • Glycosyltransferases
  • Uridine Diphosphate
  • Recycling
  • Biological Products

• Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

  Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, In : Journal of Biotechnology. 257, p. 110-117

  Research output: Contribution to journal › Article

• Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

  Research output: Contribution to journal › Article

  • Xylose
  • Uridine Diphosphate
  • Isotopes
  • Enzymes
  • Sugars

• Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

  Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, In : ChemCatChem. 9, 1, p. 161-166 6 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • sucrose
  • Sugar (sucrose)
  • microchannels
  • Microchannels

• Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

  Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, In : Journal of Biotechnology. 257, p. 131-138 8 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • Sugar (sucrose)
  • Glycerol
  • Catalyst supports
  • Enzymes

• Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

  Research output: Contribution to journal › Article

• Saccharomyces cerevisiae strain comparison in glucose-xylose fermentations on defined substrates and in high-gravity SSCF: Convergence in strain performance despite differences in genetic and evolutionary engineering history

  Novy, V., Wang, R., Westman, J. O., Franzén, C. J. & Nidetzky, B., 4 Sep 2017, In : Biotechnology for Biofuels. 10, 1, 205.

  Research output: Contribution to journal › Review article

  • Hypergravity
  • Xylose
  • Genetic Engineering
  • Yeast
  • Fermentation

• Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

  Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, In : Protein engineering, design and selection. 30, 3, p. 141-148

  Research output: Contribution to journal › Article

  • Uridine Diphosphate
  • Sugar (sucrose)
  • Nucleotides
  • Administrative data processing
  • Diphosphates

• Single-molecule study of oxidative enzymaticdeconstruction of cellulose

  Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, In : Nature Communications . 8, 7 p., 894.

  Research output: Contribution to journal › Article

  Open Access

  • cellulose
  • Cellulose
  • polysaccharides
  • Polysaccharides
  • Mixed Function Oxygenases

• Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

  Viefhues, M., Sun, S., Valikhani, D., Nidetzky, B., Vrouwe, E. X., Mayr, T. & Bolivar, J. M., 10 May 2017, In : Journal of Micromechanics and Microengineering. 27, 6, 065012.

  Research output: Contribution to journal › Article

  • Oxygen sensors
  • Immobilized Enzymes
  • Glucose Oxidase
  • Glucose oxidase
  • Optical sensors

• Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

  Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

  Research output: Contribution to journal › Article

  • Xylose
  • L-Lactate Dehydrogenase
  • Yeast
  • Fermentation
  • Glucose

• Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

  Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, In : ChemBioChem. 18, 14, p. 1387-1390 4 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • Ascorbic Acid
  • Sucrose
  • Cosmetics
  • Protonation
• 2016

  Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

  Bolivar, J. M., Eisl, I. & Nidetzky, B., 1 Jan 2016, In : Catalysis Today. 259, p. 66-80 15 p.

  Research output: Contribution to journal › Article

  • Immobilized Enzymes
  • Biocatalysts
  • Enzymes
  • Catalyst supports
  • Proteins

• Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems.

  Petschacher, B. & Nidetzky, B., 1 Apr 2016, In : Journal of Biotechnology. 235, p. 61-83 23 p.

  Research output: Contribution to journal › Article

  • Fucosyltransferases
  • Oligosaccharides
  • Human Milk
  • galactoside 3-fucosyltransferase
  • Guanosine Diphosphate Fucose

• Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

  Eibinger, M., Zahel, T., Ganner, T., Plank, H. & Nidetzky, B., 8 Mar 2016, In : Biotechnology for Biofuels . 9, 56

  Research output: Contribution to journal › Article

• Cover Image, Volume 113, Number 9, September 2016

  Bolivar, J. M., Krämer, C. E., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

  Research output: Contribution to journal › Article

• CREATING A WATER-SOLUBLE RESVERATROL-BASED ANTIOXIDANT THROUGH SITE-SELECTIVE ENZYMATIC GLUCOSYLATION

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 14 Jul 2016.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O₂-dependent enzyme

  Bolivar Bolivar, J. M., Krämer, C. E. M., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering. 113, 9, p. 1862-1872

  Research output: Contribution to journal › Article

  • Biotransformation
  • Enzymes
  • Gases
  • Immobilization
  • Oxidoreductases

• Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

  Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2 Sep 2016, In : Scientific Reports. p. 1-11 11 p.

  Research output: Contribution to journal › Article

• Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

  Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, p. 3113-3122

  Research output: Contribution to journal › Article

  • Sugar (sucrose)
  • Nucleosides
  • Sugars
  • Chromatography
  • Anions

• Exploiting the biocatalytic potential of glycosyltransferases through in situ sugar donor recycling

  Gutmann, A., Bungaruang, L., Lepak, A. & Nidetzky, B., 14 Nov 2016.

  Research output: Contribution to conference › Poster

  • Glycosyltransferases
  • Diphosphates
  • Uridine
  • Sugars
  • Recycling

• Fabrication of Cellulose Structures via Focused Electron Beam Induced Conversion: Approaching the Nanoscale

  Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2016.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Functional characterization of thenative swollenin from Trichoderma reesei:study of its possible role as C1 factor ofenzymatic lignocellulose conversion

  Eibinger, M., Sigl, K., Sattelkow, J., Ganner, T., Ramoni, J., Seiboth, B., Plank, H. & Nidetzky, B., 2016, In : Biotechnology for Biofuels . p. 9:178

  Research output: Contribution to journal › Article

• Improving the Water Solubility of Polyphenols through Site-Selective Enzymatic Glucosylation

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 12 Jun 2016.

  Research output: Contribution to conference › Poster

• Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

  Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, In : The Journal of Biological Chemistry. 291, 49, p. 25411-25426 16 p.

  Research output: Contribution to journal › Article

  • Nitriles
  • Escherichia coli
  • Oxidoreductases
  • Kinetics
  • Substrates

• Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

  Bolivar Bolivar, J. M., Tribulato, M. A., Petrasek, Z. & Nidetzky, B., 1 Nov 2016, In : Biotechnology and Bioengineering. 113, 11, p. 2342-2349 8 p.

  Research output: Contribution to journal › Article

  • Enzyme immobilization
  • Immobilization
  • Glass
  • Amino acids
  • Oxidoreductases

• Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

  Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 13 Sep 2016.

  Research output: Contribution to conference › Poster

• Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

  Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 14 Nov 2016.

  Research output: Contribution to conference › Poster

• Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

  Dewitte, G., Walmagh, M., Diricks, M., Lepak, A., Gutmann, A., Nidetzky, B. & Desmet, T., 1 Jun 2016, In : Journal of Biotechnology. 233, p. 49-55

  Research output: Contribution to journal › Article

  • Stevia
  • Glycosylation
  • Glycosyltransferases
  • Uridine Diphosphate
  • Glycosides

• Solubility Enhancement by Site-Selective Polyphenol Glucosylation

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 11 Feb 2016.

  Research output: Contribution to conference › (Old data) Lecture or Presentation