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• 2020

  A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 May 2020, In : ACS Central Science. 6, 5, p. 739-746 8 p.

  Research output: Contribution to journal › Article

  Open Access

  • Cellulose
  • Crystalline materials
  • Nanocrystals
  • Cellulases
  • Degradation

• Enzyme Immobilization in Wall-Coated Flow Microreactors

  Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

  Research output: Contribution to journal › Article

  • Immobilization
  • Enzymes
  • sucrose phosphorylase
  • Silicon Dioxide
  • Glass

• Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

  Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

  Research output: Contribution to journal › Article

  • Immobilized Enzymes
  • Proteins
  • Enzymes
  • Penicillin Amidase
  • Proton-Motive Force

• Magnetically responsive horseradish peroxidase@ZIF-8 for biocatalysis

  Ricco, R., Wied, P., Nidetzky, B., Amenitsch, H. & Falcaro, P., 28 May 2020, In : Chemical Communications. 56, 43, p. 5775-5778 4 p.

  Research output: Contribution to journal › Article

  Open Access

  • Biocatalysis
  • Horseradish Peroxidase
  • Metal Nanoparticles
  • Enzymes
  • Ions

• Model-based analysis of biocatalytic processes and performance of microbioreactors with integrated optical sensors

  Semenova, D., Fernandes, A. C., Bolivar, J. M., Rosinha Grundtvig, I. P., Vadot, B., Galvanin, S., Mayr, T., Nidetzky, B., Zubov, A. & Gernaey, K. V., 2020, In : New Biotechnology. 56, p. 27-37 11 p.

  Research output: Contribution to journal › Article

  • Microfluidics
  • Optical sensors
  • Biocatalysis
  • Glucose Oxidase
  • Screening

• On the donor substrate dependence of group‐transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase‐catalyzed transglycosylation

  Klimacek, M., Sigg, A. & Nidetzky, B., 23 Jun 2020, In : Biotechnology and Bioengineering. 2020, 117

  Research output: Contribution to journal › Article

• On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

  Bolivar, J. M. & Nidetzky, B., 1 Feb 2020, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1868, 2, 140333.

  Research output: Contribution to journal › Review article

  • Immobilized Enzymes
  • Molecules
  • Enzymes
  • Immobilization
  • Catalytic Domain

• Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose.

  Schwaiger, K. N., Voit, A., Dobiašová, H., Luley, C., Wiltschi, B. & Nidetzky, B., Nov 2020, In : Biotechnology Journal. 15, 11, 2000063.

  Research output: Contribution to journal › Article

  Open Access

  • Cellobiose
  • Plasmids
  • Enzymes
  • cellobiose phosphorylase
  • sucrose phosphorylase

• Separation behavior and microstructure of emulsified, two-phasic E. coli bioreaction mixtures

  Lauß, B., Rapp, C., Leis, D., Nidetzky, B. & Kratzer, R., 2020, In : Colloid and Interface Science Communications. 35, 100248.

  Research output: Contribution to journal › Article
• 2019

  Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

  Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • cysteine
  • Gold
  • Cysteine
  • Monolayers
  • Desorption

• A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

  Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, In : ACS Catalysis. 9, 4, p. 2962-2968 7 p.

  Research output: Contribution to journal › Article

  Open Access

  • Guanosine Diphosphate Mannose
  • Hydro-Lyases
  • Dehydration
  • Sugars
  • Deoxy Sugars

• Comparative time-lapse AFM study of crystalline cellulose deconstruction by free cellulases and the cellulosome

  Seelich, K., Eibinger, M. & Nidetzky, B., Nov 2019.

  Research output: Contribution to conference › Poster

• Correction: Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, 1 p.

  Research output: Contribution to journal › Comment/debate

  Open Access

  • Hexosaminidases
  • synthesis
  • lacto-N-neotetraose
  • Bifidobacterium bifidum

• Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

  Savino, S., Borg, A. J. E., Dennig, A., Pfeiffer, M., De Giorgi, F., Weber, H., Dubey, K. D., Rovira, C., Mattevi, A. & Nidetzky, B., 1 Dec 2019, In : Nature Catalysis. 2, 12, p. 1115-1123 9 p.

  Research output: Contribution to journal › Article

  • Biosynthesis
  • Sugars
  • Xylose
  • Uridine Diphosphate
  • Decarboxylation

• Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

  Lemmerer, M., Mairhofer, J., Lepak, A., Longus, K., Hahn, R. & Nidetzky, B., 1 Jun 2019, In : Biotechnology and Bioengineering. 116, 6, p. 1259-1268 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • Recombinant proteins
  • Glycosyltransferases
  • Cell growth
  • Recombinant Proteins
  • Escherichia coli

• Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto- N-triose II Production with Engineered exo-Hexosaminidase

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 7 Jun 2019, In : ACS Catalysis. 9, 6, p. 5503-5514 12 p.

  Research output: Contribution to journal › Article

  • Hexosaminidases
  • Glucosamine
  • Glycosylation
  • Lactose
  • Trisaccharides

• Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

  Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

  Research output: Contribution to journal › Article

  Open Access

  • Nitriles
  • Proton transfer
  • Catalysis
  • Escherichia coli
  • Protons

• Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, p. 5661-5665 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • Hexosaminidases
  • Human Milk
  • Lactose
  • Oligosaccharides
  • Glycosylation

• Preparative Asymmetric Synthesis of Canonical and Non-canonical a-amino Acids through Formal Enantioselective Biocatalytic Amination of Carboxylic Acids

  Dennig, A., Blaschke, F., Gandomkar, S., Tassano, E. & Nidetzky, B., 1 Jan 2019, In : Advanced Synthesis and Catalysis. 361, 6, p. 1348-1358 11 p.

  Research output: Contribution to journal › Article

  Open Access

  • Amination
  • Carboxylic Acids
  • Carboxylic acids
  • Amino acids
  • Fatty acids

• Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

  Zhong, C., Luley-Goedl, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology and Bioengineering. 116, 9, p. 2146-2155 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • cellodextrin phosphorylase
  • cellobiose phosphorylase
  • Polymerization
  • Solubility
  • Glucose

• Sialic acid aldolase and synthase compared in optimized multi-enzyme cascade production of 3'-sialyllactose

  Schelch, S., Gross Belduma, S. M., Eibinger, M., Kuballa, J., Petschacher, B. & Nidetzky, B., 18 Nov 2019.

  Research output: Contribution to conference › Poster

• Sustainability assessment integrated in bioprocess design in H2020 Project CARBAFIN: When thinking about process optimization - are we also thinking about environmental aspects?

  Kollmann, R., Petschacher, B., Koenig, S., Kruschitz, A., Nidetzky, B. & Passer, A., 19 Nov 2019.

  Research output: Contribution to conference › Poster

• The influence of feedstock characteristics on enzyme production in Trichoderma reesei: A review on productivity, gene regulation and secretion profiles

  Novy, V., Nielsen, F., Seiboth, B. & Nidetzky, B., 1 Jan 2019, In : Biotechnology for Biofuels. 12, 1, 238.

  Research output: Contribution to journal › Review article

  Open Access

  • Trichoderma
  • Gene expression
  • secretion
  • Feedstocks
  • Enzymes

• The microenvironment in immobilized enzymes: Methods of characterization and its role in determining enzyme performance

  Bolivar, J. M. & Nidetzky, B., 24 Sep 2019, In : Molecules . 24, 19, 3460.

  Research output: Contribution to journal › Article

  Open Access

  • Immobilized Enzymes
  • enzymes
  • Enzymes
  • Liquids
  • Ionic strength

• Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

  Zhong, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology Journal. 1900349.

  Research output: Contribution to journal › Article

  Open Access

  • Phosphorylases
  • Sucrose
  • Enzymes
  • Polymerization
  • Phosphates
• 2015

  Action of an AA9 - type lytic polysaccharide monooxygenase on the cellulose surface and its effect on cellulase hydrolytic efficiency

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 2 Aug 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

  Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, In : Analytical biochemistry. 483, p. 47-53

  Research output: Contribution to journal › Article

• Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

  Ganner, T., Sattelkow, J., Rosker, S., Eibinger, M., Chernev, B. S., Fitzek, H. M., Kraxner, J., Mayrhofer, C., Aschl, T., Nidetzky, B. & Plank, H., 2015, ASEM Workshop Advanced Electron Microscopy. ., p. 30-30

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

  Ganner, T., Eibinger, M., Rosker, S., Sattelkow, J., Aschl, T., Rattenberger, J., Fitzek, H. M., Nidetzky, B. & Plank, H., 7 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• C-Glycosyltransferases: From mechanistic insight to synthetic application in flavonoid glycosylation

  Gutmann, A., Bungaruang, L. & Nidetzky, B., 5 Aug 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

  Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, In : Analytical biochemistry. 490, p. 46-51

  Research output: Contribution to journal › Article

• Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 10 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 4 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, In : Chemical Communications. p. 3083-3086

  Research output: Contribution to journal › Article

• Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, In : ChemBioChem. 16, 13, p. 1870-1874

  Research output: Contribution to journal › Article

  • Antioxidants
  • Water
  • Solubility
  • Glycosylation
  • Glucosyltransferases

• Critical evaluation of different NDP-sugar purification strategies

  Lemmerer, M., Schmoelzer, K., Gutmann, A., Lepak, A. & Nidetzky, B., 2015.

  Research output: Contribution to conference › Poster

• Critical evaluation of different NDP-sugar purification strategies

  Lemmerer, M., Schmoelzer, K., Gutmann, A., Lepak, A. & Nidetzky, B., 2015.

  Research output: Contribution to conference › Poster

• Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

  Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dec 2015, In : Angewandte Chemie / International Edition . 54, 52, p. 15867-15871 5 p.

  Research output: Contribution to journal › Article

  • Phosphorylase Phosphatase
  • Phosphatases
  • Phosphates
  • Phosphorylation
  • Catalysts

• Expression of human sialyltransferase in yeast

  Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D., Schwab, H. & Nidetzky, B., 21 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• FUCOSYLTRANSFERASES: KEY PLAYERS IN THE BIOECHNOLOGICAL SYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES

  Petschacher, B., Kuballa, J. & Nidetzky, B., 2015.

  Research output: Contribution to conference › Poster

• Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts

  Trausinger, G., Gruber, C., Krahulec, S., Magnes, C., Nidetzky, B. & Klimacek, M., 2015, In : Biotechnology for Biofuels . 8:157, p. 1-13

  Research output: Contribution to journal › Article

• Mesoporous Silica Materials Labeled for Optical Oxygen Sensing and Their Application to Development of a Silica-Supported Oxidoreductase Biocatalyst

  Bolivar, J. M., Schelch, S., Mayr, T. & Nidetzky, B., 2 Oct 2015, In : ACS Catalysis. 5, 10, p. 5984-5993 10 p.

  Research output: Contribution to journal › Article

  • Biocatalysts
  • Silicon Dioxide
  • Oxidoreductases
  • Silica
  • Oxygen

• N-​terminally truncated glycosyltransferases for use in post-​translational sialylation

  Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent No. WO 2015001034 A1

  Research output: Patent

• Oxidation of monolignols by members of the berberine bridge enzyme family suggests a role in plant cell wall metabolism

  Daniel, B., Pavkov-Keller, T., Steiner, B., Dodic, A., Gutmann, A., Nidetzky, B., Sensen, C. W., van der Graaff, E., Wallner, S., Gruber, K. & Macheroux, P., 2015, In : The Journal of Biological Chemistry. 290, 30, p. 18770-18781

  Research output: Contribution to journal › Article

• Phosphoryl transfer from α-D-glucose 1-phosphate catalyzed by Escherichia coli sugar-phosphate phosphatases of two protein superfamily types

  Wildberger, P., Pfeiffer, M., Brecker, L., Rechberger, G. N., Birner-Gruenberger, R. & Nidetzky, B., 2015, In : Applied and Environmental Microbiology. 81, 5, p. 1559-1572 14 p.

  Research output: Contribution to journal › Article

  • glucose 1-phosphate
  • Sugar Phosphates
  • sugar phosphates
  • phosphatase
  • sugar

• Probing of the reaction pathway of human UDP-xylose synthase with site-directed mutagenesis

  Eixelsberger, T., Weber, H. & Nidetzky, B., 2015, In : Carbohydrate Research. 416, p. 1-6

  Research output: Contribution to journal › Article

• Process for the mono- and bi-​sialylation of glycoproteins employing N-​terminally truncated β-​galactoside α-​2,​6-​sialyltransferase mutants

  Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent No. WO 2015001033 A1

  Research output: Patent

• Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions

  Kratzer, R., Woodley, J. & Nidetzky, B., 2015, In : Biotechnology Advances. 33, 8, p. 1641–1652

  Research output: Contribution to journal › Article

• Saturation Transfer Difference NMR to Study Substrate and Product Binding to Human UDP-Xylose Synthase (hUXS1A) During Catalytic Event

  Puchner, C., Eixelsberger, T., Nidetzky, B. & Brecker, L., 2015, In : RSC Advances . 5, p. 86919-86926

  Research output: Contribution to journal › Article

• Speeding up the product release: a second-sphere contribution from Tyr191 to the reactivity of l-lactate oxidase revealed in crystallographic and kinetic studies of site-directed variants.

  Stoisser, T., Klimacek, M., Wilson, D. K. & Nidetzky, B., 2015, In : The FEBS journal. 282, 21, p. 4130-4140

  Research output: Contribution to journal › Article