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• 2020

  A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 May 2020, In : ACS Central Science. 6, 5, p. 739-746 8 p.

  Research output: Contribution to journal › Article

  Open Access

  • Cellulose
  • Crystalline materials
  • Nanocrystals
  • Cellulases
  • Degradation

• Enzyme Immobilization in Wall-Coated Flow Microreactors

  Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

  Research output: Contribution to journal › Article

  • Immobilization
  • Enzymes
  • sucrose phosphorylase
  • Silicon Dioxide
  • Glass

• Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

  Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

  Research output: Contribution to journal › Article

  • Immobilized Enzymes
  • Proteins
  • Enzymes
  • Penicillin Amidase
  • Proton-Motive Force

• Magnetically responsive horseradish peroxidase@ZIF-8 for biocatalysis

  Ricco, R., Wied, P., Nidetzky, B., Amenitsch, H. & Falcaro, P., 28 May 2020, In : Chemical Communications. 56, 43, p. 5775-5778 4 p.

  Research output: Contribution to journal › Article

  Open Access

  • Biocatalysis
  • Horseradish Peroxidase
  • Metal Nanoparticles
  • Enzymes
  • Ions

• Model-based analysis of biocatalytic processes and performance of microbioreactors with integrated optical sensors

  Semenova, D., Fernandes, A. C., Bolivar, J. M., Rosinha Grundtvig, I. P., Vadot, B., Galvanin, S., Mayr, T., Nidetzky, B., Zubov, A. & Gernaey, K. V., 2020, In : New Biotechnology. 56, p. 27-37 11 p.

  Research output: Contribution to journal › Article

  • Microfluidics
  • Optical sensors
  • Biocatalysis
  • Glucose Oxidase
  • Screening

• On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

  Bolivar, J. M. & Nidetzky, B., 1 Feb 2020, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1868, 2, 140333.

  Research output: Contribution to journal › Review article

  • Immobilized Enzymes
  • Molecules
  • Enzymes
  • Immobilization
  • Catalytic Domain

• Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose.

  Schwaiger, K. N., Voit, A., Dobiašová, H., Luley, C., Wiltschi, B. & Nidetzky, B., Nov 2020, In : Biotechnology Journal. 15, 11, 2000063.

  Research output: Contribution to journal › Article

  Open Access

  • Cellobiose
  • Plasmids
  • Enzymes
  • cellobiose phosphorylase
  • sucrose phosphorylase

• Separation behavior and microstructure of emulsified, two-phasic E. coli bioreaction mixtures

  Lauß, B., Rapp, C., Leis, D., Nidetzky, B. & Kratzer, R., 2020, In : Colloid and Interface Science Communications. 35, 100248.

  Research output: Contribution to journal › Article
• 2017

  An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

  Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

  Research output: Contribution to journal › Article

• A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

  Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

  Research output: Contribution to journal › Article

  • Enzyme immobilization
  • Microfluidics
  • Silicon Dioxide
  • Enzymes
  • Silica

• Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

  Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

  Research output: Contribution to journal › Article

  Open Access

  • Sugar (sucrose)
  • Nucleotides
  • Nucleosides
  • Glucose
  • Diphosphates

• Debottlenecking enzymatic production of fucosylated HMOs with prokaryotic a1,2-fucosyltransferases

  Petschacher, B., Gierlinger, K., Brunner, L., Kuballa, J. & Nidetzky, B., 23 Apr 2017.

  Research output: Contribution to conference › Poster

• Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

  Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

  Research output: Contribution to journal › Article

  • Uridine Diphosphate Glucose
  • Glycosyltransferases
  • Uridine Diphosphate
  • Recycling
  • Biological Products

• Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

  Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, In : Journal of Biotechnology. 257, p. 110-117

  Research output: Contribution to journal › Article

• Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

  Research output: Contribution to journal › Article

  • Xylose
  • Uridine Diphosphate
  • Isotopes
  • Enzymes
  • Sugars

• Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

  Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, In : ChemCatChem. 9, 1, p. 161-166 6 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • sucrose
  • Sugar (sucrose)
  • microchannels
  • Microchannels

• Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

  Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, In : Journal of Biotechnology. 257, p. 131-138 8 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • Sugar (sucrose)
  • Glycerol
  • Catalyst supports
  • Enzymes

• Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

  Research output: Contribution to journal › Article

• Saccharomyces cerevisiae strain comparison in glucose-xylose fermentations on defined substrates and in high-gravity SSCF: Convergence in strain performance despite differences in genetic and evolutionary engineering history

  Novy, V., Wang, R., Westman, J. O., Franzén, C. J. & Nidetzky, B., 4 Sep 2017, In : Biotechnology for Biofuels. 10, 1, 205.

  Research output: Contribution to journal › Review article

  • Hypergravity
  • Xylose
  • Genetic Engineering
  • Yeast
  • Fermentation

• Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

  Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, In : Protein engineering, design and selection. 30, 3, p. 141-148

  Research output: Contribution to journal › Article

  • Uridine Diphosphate
  • Sugar (sucrose)
  • Nucleotides
  • Administrative data processing
  • Diphosphates

• Single-molecule study of oxidative enzymaticdeconstruction of cellulose

  Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, In : Nature Communications . 8, 7 p., 894.

  Research output: Contribution to journal › Article

  Open Access

  • cellulose
  • Cellulose
  • polysaccharides
  • Polysaccharides
  • Mixed Function Oxygenases

• Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

  Viefhues, M., Sun, S., Valikhani, D., Nidetzky, B., Vrouwe, E. X., Mayr, T. & Bolivar, J. M., 10 May 2017, In : Journal of Micromechanics and Microengineering. 27, 6, 065012.

  Research output: Contribution to journal › Article

  • Oxygen sensors
  • Immobilized Enzymes
  • Glucose Oxidase
  • Glucose oxidase
  • Optical sensors

• Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

  Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

  Research output: Contribution to journal › Article

  • Xylose
  • L-Lactate Dehydrogenase
  • Yeast
  • Fermentation
  • Glucose

• Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

  Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, In : ChemBioChem. 18, 14, p. 1387-1390 4 p.

  Research output: Contribution to journal › Article

  • sucrose phosphorylase
  • Ascorbic Acid
  • Sucrose
  • Cosmetics
  • Protonation
• 2015

  Action of an AA9 - type lytic polysaccharide monooxygenase on the cellulose surface and its effect on cellulase hydrolytic efficiency

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 2 Aug 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

  Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, In : Analytical biochemistry. 483, p. 47-53

  Research output: Contribution to journal › Article

• Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

  Ganner, T., Sattelkow, J., Rosker, S., Eibinger, M., Chernev, B. S., Fitzek, H. M., Kraxner, J., Mayrhofer, C., Aschl, T., Nidetzky, B. & Plank, H., 2015, ASEM Workshop Advanced Electron Microscopy. ., p. 30-30

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

  Ganner, T., Eibinger, M., Rosker, S., Sattelkow, J., Aschl, T., Rattenberger, J., Fitzek, H. M., Nidetzky, B. & Plank, H., 7 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• C-Glycosyltransferases: From mechanistic insight to synthetic application in flavonoid glycosylation

  Gutmann, A., Bungaruang, L. & Nidetzky, B., 5 Aug 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

  Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, In : Analytical biochemistry. 490, p. 46-51

  Research output: Contribution to journal › Article

• Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 10 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 4 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, In : Chemical Communications. p. 3083-3086

  Research output: Contribution to journal › Article

• Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, In : ChemBioChem. 16, 13, p. 1870-1874

  Research output: Contribution to journal › Article

  • Antioxidants
  • Water
  • Solubility
  • Glycosylation
  • Glucosyltransferases

• Critical evaluation of different NDP-sugar purification strategies

  Lemmerer, M., Schmoelzer, K., Gutmann, A., Lepak, A. & Nidetzky, B., 2015.

  Research output: Contribution to conference › Poster

• Critical evaluation of different NDP-sugar purification strategies

  Lemmerer, M., Schmoelzer, K., Gutmann, A., Lepak, A. & Nidetzky, B., 2015.

  Research output: Contribution to conference › Poster

• Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

  Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dec 2015, In : Angewandte Chemie / International Edition . 54, 52, p. 15867-15871 5 p.

  Research output: Contribution to journal › Article

  • Phosphorylase Phosphatase
  • Phosphatases
  • Phosphates
  • Phosphorylation
  • Catalysts

• Expression of human sialyltransferase in yeast

  Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D., Schwab, H. & Nidetzky, B., 21 May 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• FUCOSYLTRANSFERASES: KEY PLAYERS IN THE BIOECHNOLOGICAL SYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES

  Petschacher, B., Kuballa, J. & Nidetzky, B., 2015.

  Research output: Contribution to conference › Poster

• Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts

  Trausinger, G., Gruber, C., Krahulec, S., Magnes, C., Nidetzky, B. & Klimacek, M., 2015, In : Biotechnology for Biofuels . 8:157, p. 1-13

  Research output: Contribution to journal › Article

• Mesoporous Silica Materials Labeled for Optical Oxygen Sensing and Their Application to Development of a Silica-Supported Oxidoreductase Biocatalyst

  Bolivar, J. M., Schelch, S., Mayr, T. & Nidetzky, B., 2 Oct 2015, In : ACS Catalysis. 5, 10, p. 5984-5993 10 p.

  Research output: Contribution to journal › Article

  • Biocatalysts
  • Silicon Dioxide
  • Oxidoreductases
  • Silica
  • Oxygen

• N-​terminally truncated glycosyltransferases for use in post-​translational sialylation

  Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent No. WO 2015001034 A1

  Research output: Patent

• Oxidation of monolignols by members of the berberine bridge enzyme family suggests a role in plant cell wall metabolism

  Daniel, B., Pavkov-Keller, T., Steiner, B., Dodic, A., Gutmann, A., Nidetzky, B., Sensen, C. W., van der Graaff, E., Wallner, S., Gruber, K. & Macheroux, P., 2015, In : The Journal of Biological Chemistry. 290, 30, p. 18770-18781

  Research output: Contribution to journal › Article

• Phosphoryl transfer from α-D-glucose 1-phosphate catalyzed by Escherichia coli sugar-phosphate phosphatases of two protein superfamily types

  Wildberger, P., Pfeiffer, M., Brecker, L., Rechberger, G. N., Birner-Gruenberger, R. & Nidetzky, B., 2015, In : Applied and Environmental Microbiology. 81, 5, p. 1559-1572 14 p.

  Research output: Contribution to journal › Article

  • glucose 1-phosphate
  • Sugar Phosphates
  • sugar phosphates
  • phosphatase
  • sugar

• Probing of the reaction pathway of human UDP-xylose synthase with site-directed mutagenesis

  Eixelsberger, T., Weber, H. & Nidetzky, B., 2015, In : Carbohydrate Research. 416, p. 1-6

  Research output: Contribution to journal › Article

• Process for the mono- and bi-​sialylation of glycoproteins employing N-​terminally truncated β-​galactoside α-​2,​6-​sialyltransferase mutants

  Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent No. WO 2015001033 A1

  Research output: Patent

• Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions

  Kratzer, R., Woodley, J. & Nidetzky, B., 2015, In : Biotechnology Advances. 33, 8, p. 1641–1652

  Research output: Contribution to journal › Article

• Saturation Transfer Difference NMR to Study Substrate and Product Binding to Human UDP-Xylose Synthase (hUXS1A) During Catalytic Event

  Puchner, C., Eixelsberger, T., Nidetzky, B. & Brecker, L., 2015, In : RSC Advances . 5, p. 86919-86926

  Research output: Contribution to journal › Article

• Speeding up the product release: a second-sphere contribution from Tyr191 to the reactivity of l-lactate oxidase revealed in crystallographic and kinetic studies of site-directed variants.

  Stoisser, T., Klimacek, M., Wilson, D. K. & Nidetzky, B., 2015, In : The FEBS journal. 282, 21, p. 4130-4140

  Research output: Contribution to journal › Article

• Structural and biochemical characterization of a monolignol oxidoreductase from A. thaliana implies a link between the berberine bridge enzyme-like family and cell wall metabolism

  Daniel, B., Pavkov-Keller, T., Steiner, B., Dordic, A., Gutmann, A., Nidetzky, B., Sensen, C. W., van der Graaff, E., Wallner, S., Gruber, K. & Macheroux, P., 2015.

  Research output: Contribution to conference › Poster