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  A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 May 2020, In : ACS Central Science. 6, 5, p. 739-746 8 p.

  Research output: Contribution to journal › Article

  Open Access

  • Cellulose
  • Crystalline materials
  • Nanocrystals
  • Cellulases
  • Degradation

• Acceleration of an aldo-keto reductase by minimal loop engineering

  Krump, C., Vogl, M., Brecker, L., Nidetzky, B. & Kratzer, R., 2014, In : Protein engineering, design and selection. 27, p. 245-248

  Research output: Contribution to journal › Article

• Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

  Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • cysteine
  • Gold
  • Cysteine
  • Monolayers
  • Desorption

• Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

  Bolivar, J. M., Eisl, I. & Nidetzky, B., 1 Jan 2016, In : Catalysis Today. 259, p. 66-80 15 p.

  Research output: Contribution to journal › Article

  • Immobilized Enzymes
  • Biocatalysts
  • Enzymes
  • Catalyst supports
  • Proteins

• All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

  Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, In : Analytical biochemistry. 483, p. 47-53

  Research output: Contribution to journal › Article

• Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae

  Krahulec, S., Klimacek, M. & Nidetzky, B., 2012, In : Journal of Biotechnology. 158, p. 192-202

  Research output: Contribution to journal › Article

• An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

  Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

  Research output: Contribution to journal › Article

• A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

  Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, In : ACS Catalysis. 9, 4, p. 2962-2968 7 p.

  Research output: Contribution to journal › Article

  Open Access

  • Guanosine Diphosphate Mannose
  • Hydro-Lyases
  • Dehydration
  • Sugars
  • Deoxy Sugars

• A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

  Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

  Research output: Contribution to journal › Article

  • Enzyme immobilization
  • Microfluidics
  • Silicon Dioxide
  • Enzymes
  • Silica

• A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

  Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

  Research output: Contribution to journal › Article

  • Glucose 1-Dehydrogenase
  • Mixed Function Oxygenases
  • Cytochrome P-450 Enzyme System
  • Bacillus megaterium
  • Glucose

• A two-step O- to C-glycosidic bond rearrangement using complementary glycosyltransferase activities

  Gutmann, A., Krump, C., Bungaruang, L. & Nidetzky, B., 2014, In : Chemical Communications. 50, 41, p. 5465-5468

  Research output: Contribution to journal › Article

• Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

  Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, In : ACS Applied Materials & Interfaces. 10, 8, p. 6858-6868 11 p.

  Research output: Contribution to journal › Article

  • Optical sensors
  • Proteins
  • pH sensors
  • Penicillin Amidase
  • Immobilized Enzymes

• Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

  Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

  Research output: Contribution to journal › Article

  • decarboxylation
  • Carboxy-Lyases
  • fatty acids
  • Hydrocarbons
  • Fatty acids

• Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

  Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

  Research output: Contribution to journal › Article

  Open Access

  • Sugar (sucrose)
  • Nucleotides
  • Nucleosides
  • Glucose
  • Diphosphates

• Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

  Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mar 2018, In : ChemBioChem. 19, 6, p. 596-603 8 p.

  Research output: Contribution to journal › Article

  • Lipomyces
  • Glucose-6-Phosphate
  • Phosphotransferases
  • Adenosine Diphosphate
  • Adenosine Triphosphate

• Bioprocess design guided by in situ substrate supply and product removal: Process intensification for synthesis of (S)-1-(2-chlorophenyl)ethanol

  Schmoelzer, K., Mädje, K. T., Nidetzky, B. & Kratzer, R., 2012, In : Bioresource Technology. 108, p. 216-223

  Research output: Contribution to journal › Article

• Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems.

  Petschacher, B. & Nidetzky, B., 1 Apr 2016, In : Journal of Biotechnology. 235, p. 61-83 23 p.

  Research output: Contribution to journal › Article

  • Fucosyltransferases
  • Oligosaccharides
  • Human Milk
  • galactoside 3-fucosyltransferase
  • Guanosine Diphosphate Fucose

• Candida tenuis Xylose Reductase Catalysed Reduction of Aryl Ketones for Enantioselective Synthesis of Active Pharmaceutical Ingredients

  Vogl, M., Kratzer, R., Nidetzky, B. & Brecker, L., 2012, In : Chirality. 24, p. 847-853

  Research output: Contribution to journal › Article

• Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction

  Eixelsberger, T., Brecker, L. & Nidetzky, B., 2012, In : Carbohydrate Research. 356, p. 209-214

  Research output: Contribution to journal › Article

• Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

  Eibinger, M., Zahel, T., Ganner, T., Plank, H. & Nidetzky, B., 8 Mar 2016, In : Biotechnology for Biofuels . 9, 56

  Research output: Contribution to journal › Article

• Cellulases dig deep: in situ observation of the mesoscopic structural dynamics of enzymatic cellulose degradation

  Bubner, P., Dohr, J., Plank, H., Mayrhofer, C. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, 4, p. 2759-2765

  Research output: Contribution to journal › Article

  Open Access

  File

• Cellulose surface degradation by a lytic polysaccharide monooxygenase and its effect on cellulose hydrolytic efficiency

  Eibinger, M., Ganner, T., Bubner, P., Rosker, S., Kracher, D., Haltrich, D., Ludwig, R., Plank, H. & Nidetzky, B., 2014, In : The Journal of Biological Chemistry. 289, 52, p. 35929-35938

  Research output: Contribution to journal › Article

• Characterization of a Laboratory-Scale Container for Freezing Protein Solutions with Detailed Evaluation of a Freezing Process Simulation

  Rößl, U., Jajcevic, D., Leitgeb, S., Khinast, J. & Nidetzky, B., 2013, In : Journal of Pharmaceutical Sciences . 103, 2, p. 417-426

  Research output: Contribution to journal › Article

• Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis

  Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 2013, In : Glycobiology. 23, 11, p. 1293-1304

  Research output: Contribution to journal › Article

• Co-fermentation of hexose and pentose sugars in a spent sulfite liquor matrix with genetically modified Saccharomyces cerevisiae

  Novy, V., Krahulec, S., Longus, K., Klimacek, M. & Nidetzky, B., 2013, In : Bioresource Technology. 130, p. 439-448

  Research output: Contribution to journal › Article

• Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

  Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, In : Analytical biochemistry. 490, p. 46-51

  Research output: Contribution to journal › Article

• Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation

  Krahulec, S., Kratzer, R., Longus, K. & Nidetzky, B., 2012, In : MicrobiologyOpen . 1, p. 64-70

  Research output: Contribution to journal › Article

• Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, In : Chemical Communications. p. 3083-3086

  Research output: Contribution to journal › Article

• Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes

  Brugger, D., Krondorfer, I., Zahma, K., Stoisser, T., Bolivar, J. M., Nidetzky, B., Peterbauer, C. K. & Haltrich, D., 2014, In : Biotechnology Journal. 9, 4, p. 474-482 9 p.

  Research output: Contribution to journal › Article

  • Oxidation-Reduction
  • Oxidoreductases
  • Coloring Agents
  • Oxygen
  • Enzymes

• Cover Image, Volume 113, Number 9, September 2016

  Bolivar, J. M., Krämer, C. E., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

  Research output: Contribution to journal › Article

• Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

  Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, In : ChemBioChem. 16, 13, p. 1870-1874

  Research output: Contribution to journal › Article

  • Antioxidants
  • Water
  • Solubility
  • Glycosylation
  • Glucosyltransferases

• Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

  Savino, S., Borg, A. J. E., Dennig, A., Pfeiffer, M., De Giorgi, F., Weber, H., Dubey, K. D., Rovira, C., Mattevi, A. & Nidetzky, B., 1 Dec 2019, In : Nature Catalysis. 2, 12, p. 1115-1123 9 p.

  Research output: Contribution to journal › Article

  • Biosynthesis
  • Sugars
  • Xylose
  • Uridine Diphosphate
  • Decarboxylation

• Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

  Lemmerer, M., Mairhofer, J., Lepak, A., Longus, K., Hahn, R. & Nidetzky, B., 1 Jun 2019, In : Biotechnology and Bioengineering. 116, 6, p. 1259-1268 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • Recombinant proteins
  • Glycosyltransferases
  • Cell growth
  • Recombinant Proteins
  • Escherichia coli

• Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

  Bolivar, J. M., Valikhani, D. & Nidetzky, B., 9 Aug 2018, In : Biotechnology Journal.

  Research output: Contribution to journal › Article

  • Immobilized Enzymes
  • sucrose phosphorylase
  • Enzymes
  • Biocatalysis
  • Biotransformation

• Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O₂-dependent enzyme

  Bolivar Bolivar, J. M., Krämer, C. E. M., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering. 113, 9, p. 1862-1872

  Research output: Contribution to journal › Article

  • Biotransformation
  • Enzymes
  • Gases
  • Immobilization
  • Oxidoreductases

• Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

  Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dec 2015, In : Angewandte Chemie / International Edition . 54, 52, p. 15867-15871 5 p.

  Research output: Contribution to journal › Article

  • Phosphorylase Phosphatase
  • Phosphatases
  • Phosphates
  • Phosphorylation
  • Catalysts

• Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

  Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2 Sep 2016, In : Scientific Reports. p. 1-11 11 p.

  Research output: Contribution to journal › Article

• Dissecting and Reconstructing Synergism - IN SITU VISUALIZATION OF COOPERATIVITY AMONG CELLULASES*

  Ganner, T., Bubner, P., Eibinger, M., Mayrhofer, C., Plank, H. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, 52, p. 43215-43222

  Research output: Contribution to journal › Article

• Dissecting Physical and Biochemical Factors of Catalytic Effectiveness in Immobilized D-Amino Acid Oxidase by Real-Time Sensing of O2 Availability Inside Porous Carriers

  Bolivar Bolivar, J. M., Schelch, S., Mayr, T. & Nidetzky, B., 2014, In : ChemCatChem. 6, 4, p. 981-986

  Research output: Contribution to journal › Article

  • D-Amino-Acid Oxidase
  • physical factors
  • oxidase
  • availability
  • amino acids

• Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

  Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, p. 3113-3122

  Research output: Contribution to journal › Article

  • Sugar (sucrose)
  • Nucleosides
  • Sugars
  • Chromatography
  • Anions

• Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts

  Boniello, C., Mayr, T., Bolivar Bolivar, J. M. & Nidetzky, B., 2012, In : BMC Biotechnology . 12, 11, p. 1-10

  Research output: Contribution to journal › Article

• Dynamic mechanism of proton transfer in mannitol 2-dehydrogenase from Pseudomonas fluorescens - mobile Glu292 controls proton relay through a water channel that connects the active site with bulk solvent

  Klimacek, M., Brunsteiner, M. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, 9, p. 6655-6667

  Research output: Contribution to journal › Article

• Ein Motiv im aktiven Zentrum fungiert als Schalter zwischen O‐und C‐Glykosyltransferase‐Aktivität

  Gutmann, A. & Nidetzky, B., 2012, In : Angewandte Chemie. 124, 51, p. 13051-13056

  Research output: Contribution to journal › Article

• Enzymatic C-glycosylation: Insights from the study of a complementary pair of plant O-and C-glucosyltransferases.

  Gutmann, A. & Nidetzky, B., 2013, In : Pure and applied chemistry. 85, 9, p. 1865-1877

  Research output: Contribution to journal › Article

• Enzymatic redox cascade for one-pot synthesis of uridine 5’-diphosphate xylose from uridine 5’-diphosphate glucose

  Eixelsberger, T. & Nidetzky, B., 2014, In : Advanced Synthesis & Catalysis. 356, 17, p. 3575-3584

  Research output: Contribution to journal › Article

• Enzyme Immobilization in Wall-Coated Flow Microreactors

  Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

  Research output: Contribution to journal › Article

  • Immobilization
  • Enzymes
  • sucrose phosphorylase
  • Silicon Dioxide
  • Glass

• Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

  Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

  Research output: Contribution to journal › Article

  • Nitriles
  • Imines
  • Escherichia coli
  • Amines
  • Oxidoreductases

• Examining the role of phosphate in glycosyl transfer reactions of Cellulomonas uda cellobiose phosphorylase using d-glucal as donor substrate

  Wildberger, P., Brecker, L. & Nidetzky, B., 2012, In : Carbohydrate Research. p. 224-232

  Research output: Contribution to journal › Article

• Functional characterization of thenative swollenin from Trichoderma reesei:study of its possible role as C1 factor ofenzymatic lignocellulose conversion

  Eibinger, M., Sigl, K., Sattelkow, J., Ganner, T., Ramoni, J., Seiboth, B., Plank, H. & Nidetzky, B., 2016, In : Biotechnology for Biofuels . p. 9:178

  Research output: Contribution to journal › Article

• Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

  Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

  Research output: Contribution to journal › Article

  • Uridine Diphosphate Glucose
  • Glycosyltransferases
  • Uridine Diphosphate
  • Recycling
  • Biological Products