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  • Robert Kourist
(Old data) Lecture or Presentation

Asymmetric decarboxylation of arylmalonates and racemization of profens by arylmalonate decarboxylase and its variants

Miyamoto, K., Kourist, R., Yoshida, S. & Ohta, H., 2012, p. 274-280. 7 p.

Research output: Contribution to conference(Old data) Lecture or PresentationResearchpeer-review

Article

A chemo-enzymatic tandem reaction in a mixture of deep eutectic solvent and water in continuous flow

Grabner, B., Schweiger, A. K., Gavric, K., Kourist, R. & Gruber-Wölfler, H., 2020, In : Reaction Chemistry & Engineering. 5, 2, p. 263-269

Research output: Contribution to journalArticleResearchpeer-review

Open Access

A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts

Masuch, T., Kusnezowa, A., Nilewski, S., Bautista, J. T., Kourist, R. & Leichert, L. I., 2015, In : Frontiers in Microbiology . 6

Research output: Contribution to journalArticleResearchpeer-review

Amine Transaminase from Exophiala Xenobiotica - Crystal Structure and Engineering of a Fold IV Transaminase that Naturally Converts Biaryl Ketones

Telzerow, A., Paris, J., Håkansson, M., González-Sabín, J., Ríos-Lombardía, N., Schürmann, M., Gröger, H., Morís, F., Kourist, R., Schwab, H. & Steiner, K., 1 Feb 2019, In : ACS Catalysis. 9, 2, p. 1140-1148 9 p.

Research output: Contribution to journalArticleResearchpeer-review

A Multi-Enzymatic Cascade Reaction for the Stereoselective Production of γ-Oxyfunctionalyzed Amino Acids

Enoki, J., Meisborn, J., Müller, A-C. & Kourist, R., 2016, In : Frontiers in Microbiology . 7, p. 425

Research output: Contribution to journalArticleResearchpeer-review

A One-Pot Cascade Reaction Combining an Encapsulated Decarboxylase with a Metathesis Catalyst for the Synthesis of Bio-Based Antioxidants

Gómez Baraibar, Á., Reichert, D., Mügge, C., Seger, S., Gröger, H. & Kourist, R., 14 Nov 2016, In : Angewandte Chemie / International Edition . 55, 47, p. 14823-14827 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Artificial light-harvesting complexes enable Rieske oxygenase-catalyzed hydroxylations in non-photosynthetic cells

Özgen, F. F., Runda, M. E., Burek, B. O., Wied, P., Bloh, J. Z., Kourist, R. & Schmidt, S., 18 Dec 2019, In : Angewandte Chemie / International Edition . 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Arylmalonate decarboxylase-a highly selective bacterial biocatalyst with unknown function

Miyamoto, K. & Kourist, R., Oct 2016, In : Applied Microbiology and Biotechnology. 100, 20, p. 8621-31 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Arylmalonate decarboxylase-catalyzed asymmetric synthesis of both enantiomers of optically pure flurbiprofen

Gaßmeyer, S. K., Wetzig, J., Mügge, C., Assmann, M., Enoki, J., Hilterhaus, L., Zuhse, R., Miyamoto, K., Liese, A. & Kourist, R., 2016, In : ChemCatChem. 8, p. 916-921 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

Biocatalytic strategies for the asymmetric synthesis of profens – recent trends and developments

Kourist, R., Domínguez de María, P. & Miyamoto, K., 2011, In : Green chemistry. p. 2607-2618 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Biocatalytic synthesis of optically active tertiary alcohols

Kourist, R. & Bornscheuer, U. T., 2011, In : Applied Microbiology and Biotechnology. 91, 3, p. 505-17 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants

Enoki, J., Mügge, C., Tischler, D., Miyamoto, K. & Kourist, R., 1 Apr 2019, In : Chemistry - a European Journal. 25, 19, p. 5071-5076 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Cloning and characterization of a new delta-specific L-leucine dioxygenase from Anabaena variabilis

Correia Cordeiro, R. S., Enoki, J., Busch, F., Mügge, C. & Kourist, R., 20 Oct 2018, In : Journal of Biotechnology. 284, p. 68-74 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries.

Herter, S., Nguyen, G-S., Thompson, M. L., Steffen-Munsberg, F., Schauer, F., Bornscheuer, U. T. & Kourist, R., 2011, In : Applied Microbiology and Biotechnology. 90, p. 929-939 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Creation of a lipase highly selective for trans fatty acids by protein engineering

Brundiek, H. B., Evitt, A. S., Kourist, R. & Bornscheuer, U. T., 9 Jan 2012, In : Angewandte Chemie / International Edition . 51, 2, p. 412-4 3 p.

Research output: Contribution to journalArticleResearchpeer-review

Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase

Kracht, O. N., Correia Cordeiro, R. S., Håkansson, M., Stockmann, J., Sander, D., Bandow, J., Senges, C. H. R., Logan, D. T. & Kourist, R., 20 May 2019, In : Journal of Biotechnology. 297, p. 71-77 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Draft Genome Sequence of Bordetella bronchiseptica KU1201, the First Isolation Source of Arylmalonate Decarboxylase

Yoshida, S., Enoki, J., Hemmi, R., Kourist, R., Kawakami, N. & Miyamoto, K., 7 May 2015, In : Genome Announcements. 3, 3

Research output: Contribution to journalArticleResearchpeer-review

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Miyauchi, Y., Kourist, R., Uemura, D. & Miyamoto, K., 2011, In : Chemical Communications. 47, p. 7503-7505 3 p.

Research output: Contribution to journalArticleResearchpeer-review

Durch Trennen verbinden

Schmidt, S., Gröger, H. & Kourist, R., Oct 2017, In : Nachrichten aus der Chemie. 65, 10, p. 985-988 4 p.

Research output: Contribution to journalArticleResearch

Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance

Yoshida, S., Enoki, J., Kourist, R. & Miyamoto, K., 2015, In : Bioscience, Biotechnology and Biochemistry. 79, 12, p. 1965-1971 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Engineering the promiscuous racemase activity of an arylmalonate decarboxylase

Kourist, R., Miyauchi, Y., Uemura, D. & Miyamoto, K., 10 Jan 2011, In : Chemistry - a European Journal. 17, 2, p. 557-63 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzymatic decarboxylation—an emerging reaction for chemicals production from renewable resources

Kourist, R., Guterl, J-K., Miyamoto, K. & Sieber, V., 2014, In : ChemCatChem. 6, 3, p. 689-701 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803

Böhmer, S., Köninger, K., Gomez-Baraibar, A., Bojarra, S., Mügge, C., Schmidt, S., Nowaczyk, M. & Kourist, R., Aug 2017, In : Catalysts. 7, 8, 8 p., 240.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Expression of enantioselective enzymes in cyanobacteria

Bartsch, M., Gaßmeyer, S. K., Igarashi, K., Miyamoto, M., Rögner, M., Nowaczyc, M. & Kourist, R., 2015, In : Microbial cell factories . 14, p. 53 1 p.

Research output: Contribution to journalArticleResearchpeer-review

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Kourist, R., Bracharz, F., Lorenzen, J., Kracht, O. N., Chovatia, M., Daum, C., Deshpande, S., Lipzen, A., Nolan, M., Ohm, R. A., Grigoriev, I. V., Sun, S., Heitman, J., Brück, T. & Nowrousian, M., 21 Jul 2015, In : mBio. 6, 4, p. e00918

Research output: Contribution to journalArticleResearchpeer-review

Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Assil Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 19 Mar 2019, In : ChemSusChem. 12, 11, p. 2361-2365 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases

Schrepfer, P., Buettner, A., Goerner, C., Hertel, M., van Rijn, J., Wallrapp, F., Eisenreich, W., Sieber, V., Kourist, R. & Brück, T., 23 Feb 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 8, p. E958-67

Research output: Contribution to journalArticleResearchpeer-review

Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining

Nguyen, G-S., Thompson, M. L., Grogan, G., Bornscheuer, U. T. & Kourist, R., 2011, In : Journal of molecular catalysis / B. 70, p. 88-94 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Lichtgetriebene Ganzzellbiotransformation mit rekombinanten Cyanobakterien

Nowaczyk, M. & Kourist, R., 2016, In : Biospektrum. 22, 7, p. 765-767 3 p.

Research output: Contribution to journalArticleResearch

Light-driven Enzymatic Decarboxylation

Köninger, K., Grote, M., Zachos, I., Hollmann, F. & Kourist, R., 22 May 2016, In : Journal of visualized experiments . 111

Research output: Contribution to journalArticleResearchpeer-review

Lipases as Sustainable Biocatalysts for the Sustainable Industrial Production of Fine Chemicals and Cosmetics

Kourist, R., Hollmann, F. & Nguyen, G. S., 2014, In : JSM Biotechnology & Biomedical Engineering. 2, p. 1029 1 p.

Research output: Contribution to journalArticleResearchpeer-review

One-Pot Transformation of Ketoximes into Optically Active Alcohols and Amines by Sequential Action of Laccases and Ketoreductases or ω-Transaminases

Correia Cordeiro, R. S., Ríos-Lombardía, N., Morís, F., Kourist, R. & González-Sabín, J., 20 Feb 2019, In : ChemCatChem. 11, 4, p. 1272-1277 6 p.

Research output: Contribution to journalArticleResearchpeer-review

One-step enzyme extraction and immobilization for biocatalysis applications.

Cassimjee, K. E., Kourist, R., Lindberg, D., Wittrup Larsen, M., Thanh, N. H., Widersten, M., Bornscheuer, U. T. & Berglund, P., 2011, In : Molecular biotechnology. 6, p. 463-469 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Overcoming the incompatibility challenge in chemo-enzymatic and multi-catalytic cascade reactions.

Schmidt, S., Castiglione, K. & Kourist, R., 6 Sep 2017, In : Chemistry - a European Journal.

Research output: Contribution to journalArticleResearchpeer-review

Photobiocatalytic decarboxylation for olefin synthesis

Zachos, I., Gaßmeyer, S. K., Bauer, D., Sieber, V., Hollmann, F. & Kourist, R., 2015, In : Chemical Communications. 51, p. 1918-1921 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Photosynthetic production of enantioselective biocatalysts

Bartsch, M., Gassmeyer, S. K., Köninger, K., Igarashi, K., Liauw, P., Dyczmons-Nowaczyk, N., Miyamoto, K., Nowaczyk, M. M. & Kourist, R., 15 Apr 2015, In : Microbial cell factories . 14, p. 53

Research output: Contribution to journalArticleResearchpeer-review

Preparation of optically pure flurbiprofen via an integrated chemo-enzymatic synthesis pathway

Enoki, J., Linhorst, M., Busch, F., Baraibar, Á. G., Miyamoto, K., Kourist, R. & Mügge, C., 1 Apr 2019, In : Molecular Catalysis. 467, p. 135-142 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Production of macrocyclic sesqui‐and diterpenes in heterologous microbial hosts: A systems approach to harness Nature’s molecular diversity

Brück, T., Kourist, R. & Loll, B., 2014, In : ChemCatChem. 6, 5, p. 1142-1165 24 p.

Research output: Contribution to journalArticleResearchpeer-review

Protein engineering of arylmalonate decarboxylase G74C, an artificial racemase

Kourist, R. & Miyamoto, K. ., 2011, In : Biotechnology International. 23, p. 6-9 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols

Rehdorf, J., Behrens, G. A., Nguyen, G-S., Kourist, R. & Bornscheuer, U. T., Feb 2012, In : Applied Microbiology and Biotechnology. 93, 3, p. 1119-26 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Raman Microspectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells

Aljakouch, K., Lechtonen, T., Yosef, H. K., Hammoud, M. K., Alsaidi, W., Kötting, C., Mügge, C., Kourist, R., El-Mashtoly, S. F. & Gerwert, K., 11 Jun 2018, In : Angewandte Chemie / International Edition . 57, 24, p. 7250-7254 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Recombinant Cyanobacteria for the Asymmetric Reduction of C=C Bonds Fueled by the Biocatalytic Oxidation of Water

Köninger, K., Gómez Baraibar, Á., Mügge, C., Paul, C. E., Hollmann, F., Nowaczyk, M. M. & Kourist, R., 25 Apr 2016, In : Angewandte Chemie / International Edition . 55, 18, p. 5582-5 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Semiempirical QM/MM calculations reveal a step-wise proton transfer and an unusual thiolate pocket in the mechanism of the racemizing mutant G74C of arylmalonate decarboxylase

Busch, F., Hülsemann, N., Enoki, J., Miyamoto, K., Bocola, M. & Kourist, R., 2016, In : Catalysis Science & Technology. 6, p. 4937-4944 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Sequence-Based Screening for Rare Enzymes: New Insights into the World of AMDases Reveal a Conserved Motif and 58 Novel Enzymes Clustering in Eight Distinct Families

Maimanakos, J., Chow, J., Gaßmeyer, S. K., Güllert, S., Busch, F., Kourist, R. & Streit, W. R., 2016, In : Frontiers in Microbiology . 7, p. 1332

Research output: Contribution to journalArticleResearchpeer-review

STD-NMR-Based Protein Engineering of the Unique Arylpropionate-Racemase AMDase G74C

Gaßmeyer, S. K., Yoshikawa, H., Enoki, J., Hülsemann, N., Stoll, R., Miyamoto, K. & Kourist, R., 23 Jun 2015, In : ChemBioChem. 16, 3, p. 1943-1949

Research output: Contribution to journalArticleResearchpeer-review

Stereoselective Biotransformations of Cyclic Imines in Recombinant Cells of Synechocystis sp. PCC 6803

Buechsenschuetz, H. C., Vidimce-Risteski, V., Eggbauer, B., Schmidt, S., Winkler, C. K., Schrittwieser, J. H., Kroutil, W. & Kourist, R., 2019, In : ChemCatChem. 12, 3, p. 726-730

Research output: Contribution to journalArticleResearchpeer-review

Thermally driven asymmetric domino reaction catalyzed by a thermostable esterase and its variants

Wada, R., Kumon, T., Kourist, R., Ohta, H., Uemura, D., Yoshida, S. & Miyamoto, K., 2013, In : Tetrahedron letters. 54, 15, p. 1921-1923 3 p.

Research output: Contribution to journalArticleResearchpeer-review