Find Research Outputs — Graz University of Technology

2017

Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

Research output: Contribution to journal › Article

2012

Switching between O‐and C‐Glycosyltransferase through Exchange of Active‐Site Motifs

Gutmann, A. & Nidetzky, B., 2012, In : Angewandte Chemie / International Edition . 51, 51, p. 12879-12883

Research output: Contribution to journal › Article

2018

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, In : Microbial cell factories . 17, 1, 59.

Research output: Contribution to journal › Article

2012

Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction

Eixelsberger, T., Brecker, L. & Nidetzky, B., 2012, In : Carbohydrate Research. 356, p. 209-214

Research output: Contribution to journal › Article

2018

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar, S., Bernal, C., Bolivar, J. M., Nidetzky, B., López-Gallego, F. & Mesa, M., 1 Apr 2018, In : Molecular Catalysis. p. 106-113 8 p.

Research output: Contribution to journal › Article

2016

Cover Image, Volume 113, Number 9, September 2016

Bolivar, J. M., Krämer, C. E., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article

2018

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

Research output: Contribution to journal › Article

2012

Employing disulphide bond mapping to investigate the aggregation mechanism of rhG-CSF

Rößl, U., Wiesbauer, J., Leitgeb, S., Birner-Grünberger, R. & Nidetzky, B., 2012.

Research output: Contribution to conference › Poster

2016

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

Dewitte, G., Walmagh, M., Diricks, M., Lepak, A., Gutmann, A., Nidetzky, B. & Desmet, T., 1 Jun 2016, In : Journal of Biotechnology. 233, p. 49-55

Research output: Contribution to journal › Article

2014

Glycosyltransferases: On the way towards industrial biocatalysts

Schmoelzer, K., Gutmann, A. & Nidetzky, B., 16 Dec 2014.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2016

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

Schmoelzer, K., Gutmann, A., Diricks, M., Desmet, T. & Nidetzky, B., 2016, In : Biotechnology Advances. 34, 2, p. 88-111

Research output: Contribution to journal › Article

The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production

Novy, V., Schmid, M., Eibinger, M., Petrasek, Z. & Nidetzky, B., 9 Aug 2016, In : Biotechnology for Biofuels. 9, 1, 169.

Research output: Contribution to journal › Article

2014

Enzymatic redox cascade for synthesis of nucleotide sugars

Eixelsberger, T., Horvat, D. & Nidetzky, B., 2014.

Research output: Contribution to conference › Poster

2012

Stability of L-Lactic Dehydrogenase in a Lab-Scale Freezing Container

Rößl, U., Jajcevic, D., Leitgeb, S. & Nidetzky, B., 2012.

Research output: Contribution to conference › Poster

2019

Correction: Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, 1 p.

Research output: Contribution to journal › Comment/debate

Open Access

2016

Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, In : The Journal of Biological Chemistry. 291, 49, p. 25411-25426 16 p.

Research output: Contribution to journal › Article

2018

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

Research output: Contribution to journal › Article

2012

Aggregation of hGH - Insights by accelerated stress studies and reversible refolding

Wiesbauer, J., Boeszoermenyi, A. & Nidetzky, B., 2012.

Research output: Contribution to conference › Poster

2018

Process intensification for O₂-dependent enzymatic transformations in continuous single-phase pressurized flow

Bolivar, J. M., Mannsberger, A., Thomsen, M. S., Tekautz, G. & Nidetzky, B., 4 Dec 2018, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article

2014

Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis, carrying a natural Ser-to-Thr substitution

Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 2014.

Research output: Contribution to conference › Poster

Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes

Brugger, D., Krondorfer, I., Zahma, K., Stoisser, T., Bolivar, J. M., Nidetzky, B., Peterbauer, C. K. & Haltrich, D., 2014, In : Biotechnology Journal. 9, 4, p. 474-482 9 p.

Research output: Contribution to journal › Article

A two-step O- to C-glycosidic bond rearrangement using complementary glycosyltransferase activities

Gutmann, A., Krump, C., Bungaruang, L. & Nidetzky, B., 2014, In : Chemical Communications. 50, 41, p. 5465-5468

Research output: Contribution to journal › Article

2016

Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 14 Nov 2016.

Research output: Contribution to conference › Poster

2014

Surface structural dynamics of enzymatic cellulose degradation, revealed by combined kinetic and atomic force microscopy studies

Eibinger, M., Bubner, P., Ganner, T., Plank, H. & Nidetzky, B., 2014, In : The FEBS journal. 281, p. 275-290

Research output: Contribution to journal › Article

2016

Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems.

Petschacher, B. & Nidetzky, B., 1 Apr 2016, In : Journal of Biotechnology. 235, p. 61-83 23 p.

Research output: Contribution to journal › Article

2012

Ein Motiv im aktiven Zentrum fungiert als Schalter zwischen O‐und C‐Glykosyltransferase‐Aktivität

Gutmann, A. & Nidetzky, B., 2012, In : Angewandte Chemie. 124, 51, p. 13051-13056

Research output: Contribution to journal › Article

2014

High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

Ribitsch, D., Zitzenbacher, S., Augustin, P. A., Schmoelzer, K., Luley, C., Thomann, M., Jung, C., Sobek, H., Müller, R., Nidetzky, B. & Schwab, H., 2014, In : Microbial cell factories . 13, p. 138-149

Research output: Contribution to journal › Article

2019

Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

Zhong, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology Journal. 1900349.

Research output: Contribution to journal › Article

Open Access

Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto- N-triose II Production with Engineered exo-Hexosaminidase

Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 7 Jun 2019, In : ACS Catalysis. 9, 6, p. 5503-5514 12 p.

Research output: Contribution to journal › Article

2012

Investigating Freeze and Thaw Behavior of Protein Solutions using a Lab-Scale Freezing Container

Rößl, U., Jajcevic, D., Leitgeb, S. & Nidetzky, B., 4 May 2012.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2014

Multi-enzymatic synthesis of UDP-xylose

Eixelsberger, T. & Nidetzky, B., 13 Feb 2014.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2020

On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

Bolivar, J. M. & Nidetzky, B., 1 Feb 2020, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1868, 2, 140333.

Research output: Contribution to journal › Review article

Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

Research output: Contribution to journal › Article

2012

Non-native aggregation of recombinant human granulocyte-colony stimulating factor under simulated process stress conditions

Rößl, U., Wiesbauer, J., Leitgeb, S., Birner-Grünberger, R. & Nidetzky, B., 2012, In : Biotechnology Journal. 7, 8, p. 1014-1024

Research output: Contribution to journal › Article

Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

Kratzer, R., Mädje, K. T. C. & Nidetzky, B., 6 Nov 2012.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2017

Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

Research output: Contribution to journal › Article

2014

Acceleration of an aldo-keto reductase by minimal loop engineering

Krump, C., Vogl, M., Brecker, L., Nidetzky, B. & Kratzer, R., 2014, In : Protein engineering, design and selection. 27, p. 245-248

Research output: Contribution to journal › Article

2012

NMR studies of ternary carbohydrate - NAD(H)- Candida tenuis xylose reductase complexes

Vogl, M., Kratzer, R., Nidetzky, B. & Brecker, L., 22 Apr 2012.

Research output: Contribution to conference › (Old data) Lecture or Presentation

Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

Mädje, K. T., Schmoelzer, K., Nidetzky, B. & Kratzer, R., 2012, In : Microbial cell factories . 11, p. 1-7

Research output: Contribution to journal › Article

2014

Dissecting Physical and Biochemical Factors of Catalytic Effectiveness in Immobilized D-Amino Acid Oxidase by Real-Time Sensing of O2 Availability Inside Porous Carriers

Bolivar Bolivar, J. M., Schelch, S., Mayr, T. & Nidetzky, B., 2014, In : ChemCatChem. 6, 4, p. 981-986

Research output: Contribution to journal › Article

2016

β-Cyclodextrin Improves Solubility and Enzymatic C-Glucosylation of the Flavonoid Phloretin

Bungaruang, L., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, 3, p. 486-493

Research output: Contribution to journal › Article

2017

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

Research output: Contribution to journal › Article

2012

Structural and kinetic evidence that the catalytic reaction of human UDP-glucose 6-dehydrogenase involves covalent thiohemiacetal and thioester enzyme intermediates

Egger, S., Chaikuad, A., Klimacek, M., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, p. 2119-2129

Research output: Contribution to journal › Article

2014

Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

Klimacek, M., Kirl, E., Krahulec, S., Longus, K., Novy, V. & Nidetzky, B., 2014, In : Microbial cell factories . 13, 37, p. 1-12

Research output: Contribution to journal › Article

2012

Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid

Eixelsberger, T., Sykora, S., Egger, S., Brunsteiner, M., Kavanagh, K., Oppermann, U., Brecker, L. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, 37, p. 31349-31358

Research output: Contribution to journal › Article

2019

Sialic acid aldolase and synthase compared in optimized multi-enzyme cascade production of 3'-sialyllactose

Schelch, S., Gross Belduma, S. M., Eibinger, M., Kuballa, J., Petschacher, B. & Nidetzky, B., 18 Nov 2019.

Research output: Contribution to conference › Poster

2012

Structure and mechanism of human UDP-xylose synthase

Eixelsberger, T., Sykora, S., Egger, S., Brunsteiner, M., Kavanagh, K., Oppermann, U., Brecker, L. & Nidetzky, B., 21 May 2012.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2017

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

Research output: Contribution to journal › Article

2020

On the donor substrate dependence of group‐transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase‐catalyzed transglycosylation

Klimacek, M., Sigg, A. & Nidetzky, B., 23 Jun 2020, In : Biotechnology and Bioengineering. 2020, 117

Research output: Contribution to journal › Article

A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 May 2020, In : ACS Central Science. 6, 5, p. 739-746 8 p.

Research output: Contribution to journal › Article

Open Access

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